共培养系统在软骨组织工程中的应用和发展

发布时间：2018-01-20 06:29

本文关键词： 软骨细胞间充质干细胞生物相容性材料组织工程组织构建软骨组织工程共培养生物材料国家自然科学基金　出处：《中国组织工程研究》2017年12期 　论文类型：期刊论文

【摘要】：背景:近年来大量的研究证明,共培养应用到软骨组织工程中能够成功构建具有更好生物学特性的组织工程软骨。目的:通过对共培养概念的提出及共培养系统中种子细胞来源、细胞混合比率、共培养空间模式及生物材料各方面分别进行整理和深入阐述,归纳分析各因素对组织工程软骨生物学特性的效应,展望未来共培养系统在组织工程软骨研究中的发展方向。方法:第一作者检索1976年1月至2016年5月Pub Med数据库、Web of Science数据库和中国知网中的相关文献,检索关键词为"Co-culture,Co-culture systems;articular cartilage,chondrocytes,mesenchymal stem cells;tissue engineering,articular cartilage tissue engineering;共培养,共培养系统;关节软骨,软骨细胞,间充质干细胞;组织工程,软骨组织工程"。最后纳入文献共计60篇,中文1篇,英文59篇。结果与结论:(1)共培养强调了细胞处理过程中生物微环境的重要性,即在体外为细胞生长提供物理-化学-生物三位一体的刺激因素;(2)在软骨组织工程中,共培养很好地维持了软骨细胞的活性和天然细胞表型,能够诱导具有多项分化潜能的间充质干细胞分化成软骨。另外,为软骨组织工程软骨细胞的来源受限问题和骨软骨复合伤的治疗带来新的途径;(3)但是共培养中细胞间相互作用的具体机制仍然有待进一步研究,从而为优化共培养系统培养条件提供有力依据,以更好地应用到软骨组织工程中构建优良的组织工程软骨组织。
[Abstract]:Background: in recent years, a large number of studies have proved. Co-culture can successfully construct tissue engineering cartilage with better biological characteristics. Objective: to propose the concept of co-culture and the source of seed cells in co-culture system. Cell mixing ratio, co-culture space model and biomaterials were summarized and discussed in detail, and the effects of various factors on the biological characteristics of tissue engineered cartilage were summarized and analyzed. Methods: Pub Med database was searched from January 1976 to May 2016. Web of Science database and related literature in China, the key word is "Co-cultureof Co-systems"; Articular cartilage chondrocytes mesenchymal stem cells; Tissue engineering cartilage tissue engineering; Co-culture, co-culture system; Articular cartilage, chondrocytes, mesenchymal stem cells; Tissue engineering, cartilage tissue engineering ". Finally, 60 papers were included, 1 in Chinese and 59 in English. Results and conclusion: 1) Co-culture emphasized the importance of biological microenvironment in the process of cell processing. That is, in vitro for cell growth to provide a physical-chemical-biological trinity stimulating factors; (2) in cartilage tissue engineering, co-culture can maintain the activity of chondrocytes and phenotype of natural cells, and induce the differentiation of mesenchymal stem cells with multiple differentiation potential into cartilage. It provides a new approach for the treatment of cartilage tissue engineering chondrocytes and the treatment of osteochondral complex injury. However, the specific mechanism of intercellular interaction in co-culture still needs to be further studied, so as to provide a strong basis for optimizing the culture conditions of co-culture system. In order to better use in cartilage tissue engineering to construct good tissue engineering cartilage tissue.
【作者单位】：解放军总医院骨科研究所骨科再生医学北京市重点实验室全军骨科战创伤重点实验室;解放军总医院麻醉科;安徽省立医院骨二科;
【基金】：国家自然科学基金资助项目(81472092) 国家高技术发展研究计划(863计划)资助项目(2015AA020303)~~
【分类号】：R318.08;R68
【正文快照】： 文章快速阅读:软骨组织工程中的共培养系统细胞混合比率最优细胞混合比率的共培养系统度析维剖直接或间接共培养共培养细胞作用模式共培养系统在软骨构思、设计多构组织工程中的研究、结生物材料的作用选择合适生物材料软骨组织工程研究次与层分多成平面或三维细胞培养立体培

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