七氟醚对幼鼠海马区生长相关蛋白-43和神经细胞粘附分子表达的影响

发布时间：2018-01-24 10:23

本文关键词： 七氟醚认知障碍突触可塑性生长相关蛋白-43 神经细胞粘附分子　出处：《新疆医科大学》2015年硕士论文　论文类型：学位论文

【摘要】：目的：探讨七氟醚麻醉对幼鼠认知功能及海马区生长相关蛋白(GAP-43)、神经细胞粘附分子(NCAM)表达的影响。方法：实验用7日龄SD大鼠45只,雌雄不拘,体重15-20g,采用随机数字表法分为5组(11=9)：L1组和L2组分别吸入1.5%七氟醚2h和6h；Hl组和H2组分别吸入3%七氟醚2h和6h；C组吸入30%氧气。待大鼠生长至出生后14d时开始Morris水迷宫训练7天,测定其定位航行和空间探索能力。水迷宫测试结束后断头取大鼠海马组织,采用Western blot法检测大鼠海马神经元GAP-43和NCAM蛋白表达。结果：1.与C组比较,L2、H1、H2组大鼠平均逃避潜伏期延长,平台象限停留时间缩短,三组大鼠海马区GAP-43表达水平均下降,L2组和H2组穿越平台次数减少(P0.05),L1组各项指标与C比较差异无统计学意义(P0.05)；2.五组大鼠海马区NCAM表达结果差异无统计学意义(P0.05)。结论：1.5%七氟醚2h麻醉对7d大鼠发育期认知功能无明显影响,但1.5%七氟醚6h麻醉及3%七氟醚麻醉对大鼠认知功能有影响,可能与海马区GAP-43表达水平降低有关,七氟醚麻醉对新生大鼠海马NCAM表达无影响。
[Abstract]:Objective: to investigate the effects of sevoflurane anesthesia on cognitive function and hippocampal growth related protein (GAP-43) in young rats. Methods: 45 SD rats aged 7 days, male and female, weighing 15-20 g. Five groups were randomly divided into 5 groups: group L 1 and group L 2 inhaled 1.5% sevoflurane for 2 h and 6 h, respectively. 3% sevoflurane was inhaled for 2 h and 6 h in H _ 1 and H _ 2 groups, respectively. Group C inhaled 30% oxygen and began Morris water maze training for 7 days when the rats grew to 14 days after birth. At the end of the water maze test, the hippocampal tissue of the rats was removed. Western blot method was used to detect the expression of GAP-43 and NCAM protein in hippocampal neurons of rats. The average escape latency was prolonged and the platform quadrant residence time was shortened in H2 group. The expression of GAP-43 in hippocampal region of the three groups decreased significantly (P 0.05). There was no significant difference between L1 group and C group (P 0.05). 2. There was no significant difference in the expression of NCAM in hippocampal area among the five groups. Conclusion: 1. 1. 5% sevoflurane anesthesia for 2 h has no significant effect on cognitive function of 7 d rats. However, 1.5% sevoflurane anesthesia for 6 h and 3% sevoflurane anesthesia affected the cognitive function of rats, which may be related to the decrease of GAP-43 expression in hippocampus. Sevoflurane anesthesia had no effect on the expression of NCAM in hippocampus of neonatal rats.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R614.21

【相似文献】