右美托咪定对神经病理性痛大鼠脊髓背角COX-2和c-fos表达的影响

发布时间：2018-01-30 02:31

本文关键词： 右美托咪定神经病理性痛大鼠环氧化酶- c-fos　出处：《中国现代医学杂志》2017年27期 　论文类型：期刊论文

【摘要】：目的探讨右美托咪定对神经病理性痛大鼠脊髓背角COX-2和c-fos表达的影响。方法90只健康成年雄性Wistar大鼠随机分为假手术组(n=30)、神经病理性痛组(n=30)和右美托咪定组(n=30),利用坐骨神经结扎的方法复制神经病理性痛模型,右美托咪定组大鼠于术后即刻开始至处死前1天,每天腹腔注射50μg/kg右美托咪定,1次/d,其他两组大鼠给予等容量生理盐水腹腔注射。分别于术前1 d(T_0)、术后3 d(T_1)、术后7 d(T_2)和术后14 d(T_3)对造模大鼠热痛阈(TWL)和机械痛阈(MWT)进行测定,分别于T_1、T_2和T_3时测定完TWL和MWT后,处死并取脊髓组织,利用实时荧光定量PCR检测大鼠脊髓背角中COX-2 m RNA和c-fos m RNA表达。结果与假手术组比较,神经病理性痛组和右美托咪定组大鼠T_1~T_3时TWL均缩短,MWT均降低,差异有统计学意义(P0.05),与神经病理性痛组比较,右美托咪定组大鼠T_1~T_3时TWL均延长,而MWT均升高,差异有统计学意义(P0.05);与假手术组比较,神经病理性痛组和右美托咪定组大鼠T_1~T_3时COX-2 m RNA相对表达量和c-fos m RNA相对表达量均升高,差异均有统计学意义(P0.05),与神经病理性痛组相比,右美托咪定组大鼠T_1~T_3时COX-2 m RNA相对表达量和c-fos m RNA相对表达量均降低,差异有统计学意义(P0.05)。结论右美托咪定可有效抑制神经病理性痛大鼠中枢痛觉敏化,其机制可能与下调脊髓背角COX-2和c-fos表达有关。
[Abstract]:Objective to investigate the effect of dexmetomidine on the expression of COX-2 and c-fos in spinal dorsal horn of neuropathic pain rats. Methods 90 healthy adult male Wistar rats were randomly divided into sham-operated group. ). Neuropathic pain model was established by ligation of sciatic nerve in neuropathic pain group (n = 30) and dexmetomidine group (n = 30). The rats in the dexmetomidine group were injected intraperitoneally with 50 渭 g / kg dexmetomidine once a day until the first day before their death. The other two groups were given intraperitoneal injection of normal saline of equal volume. The thermal pain threshold (TWL) and the mechanical pain threshold (MWTT) of the model rats were measured at T1. After TWL and MWT were measured at the time of T _ 2 and T _ s _ 3, the spinal cord tissues were killed and taken. The expression of COX-2 m RNA and c-fos m RNA in the spinal dorsal horn of rats was detected by real-time fluorescence quantitative PCR. In the neuropathic pain group and dexmetomidine group, the TWL shortened and decreased at the time of T _ 1 / T _ 3, and the difference was statistically significant (P 0.05), compared with the neuropathic pain group. In the right metoimidine group, the TWL was prolonged and the MWT was increased at the time of T _ S _ 1 and T _ 3. The difference was statistically significant (P 0.05). Compared with sham operation group, the relative expression of COX-2 m RNA and c-fos m RNA in neuropathic pain group and dexmetomidine group were increased at T _ 1 and T _ 3. The difference was statistically significant (P 0.05), compared with the neuropathic pain group. The relative expression of COX-2 m RNA and c-fos m RNA were decreased in the right metoimidine group. Conclusion dexmetomidine can effectively inhibit central pain sensitization in neuropathic pain rats. The mechanism may be related to the down-regulation of the expression of COX-2 and c-fos in the dorsal horn of spinal cord.
【作者单位】：石河子大学第一附属医院重症医学一科;石河子大学第一附属医院骨二科;
【基金】：国家自然科学基金(No:81360185)
【分类号】：R614
【正文快照】： 右美托咪定是一种美托咪定的右旋异构体,可高选择性激动α2肾上腺素能受体,在镇痛、镇静及抗交感神经活性等方面发挥重要作用[1],有研究指出[2],右美托咪定可有效缓解神经病理性疼痛。但具体作用机制尚未完全研究清楚。有研究指出[3],外周神经发生损伤后,环氧化酶2(cyclooxyge

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