兔骨髓脂肪诱发脂肪栓塞综合征动物模型的建立方法探讨

发布时间：2018-01-30 17:19

本文关键词： 脂肪栓塞综合征骨髓脂肪动物模型兔　出处：《中国矫形外科杂志》2017年10期 　论文类型：期刊论文

【摘要】：[目的]探讨骨折并发脂肪栓塞综合征(FES)动物模型的建立方法和影响因素。[方法]32只新西兰大白兔随机分为模型组(A、B、C组)和对照组(D组),A、B、C组以0.2 ml/min经股静脉注入0.2、0.4、0.8 ml/kg骨髓脂肪,D组注入生理盐水。在注射前(0 h)及注射后1、2、4、6、8 h,行血气分析和血常规检查。注射后8 h处死,观察肺大体改变并行HE、油红O染色。[结果](1)与对照组比较,模型组0.5 h内均出现了呼吸急促等表现,期间B组死亡3只(P0.05),C组均死亡(P0.01),A、D两组无死亡;(2)与D组比较,A组动脉血氧分压(PaO_2)最低值为(10.79±1.16)kPa(P0.05),B组为(6.78±0.93)kPa(P0.01);(3)注射8 h后,B组肺大体见暗红色肝样变,HE染色见脂肪空泡形成,油红O染色见特异性橘红色脂滴。[结论]B组兔在注射骨髓脂肪2 h后成功地建立了类似骨折并发FES的模型,成功率85%。
[Abstract]:[Objective] to explore the method and influencing factors of the animal model of fracture complicated with fat embolism syndrome (FESs). [Methods: Thirty-two New Zealand white rabbits were randomly divided into two groups: the model group (group A) and the control group (group D). Group D was injected with normal saline at 0 ml/kg. Blood gas analysis and routine examination were performed at 0 h before injection and 6 hours after injection. The rats were killed 8 hours after injection. The changes of lung were observed with HEY and oil red O staining. [Results: compared with the control group, the model group showed shortness of breath within 0.5 h. During the period, 3 rats died in group B and all died in group C (P 0.01). Group D had no death; (2) compared with group D, the lowest value of Pao _ 2 in group A was 10.79 卤1.16kPaP0.05). In group B, it was 6.78 卤0.93 KPA (P 0.01); 3) in group B, fat vacuoles were found in the lungs of group B after 8 hours of injection, and fat vacuoles were found in the lungs of group B with HE staining and specific orange fat drops with oil red O staining. [Conclusion: group B rabbits successfully established the model of similar fracture complicated with FES 2 h after injection of bone marrow fat, the success rate was 85%.
【作者单位】：南方医科大学;中国人民解放军武汉总医院骨科;
【基金】：湖北省卫计委重点科研项目(编号:WJ2015MA013)
【分类号】：R-332;R683
【正文快照】： 骨折并发脂肪栓塞综合征(fat embolism syn-drome,FES)是指骨折后骨髓腔内脂质成分入血阻塞于微循环而引起的一系列病理生理改变的临床综合征[1]。其特点是发病急、进展快,目前尚缺乏特异性治疗药物,严重威胁创伤骨折患者的生命。FES发病机制及防治方法多年来尚未取得突破性

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