基于软骨细胞外基质的取向支架的制备及评价

发布时间：2018-01-31 18:09

本文关键词： 软骨细胞外基质取向支架组织工程定向结晶冷冻干燥　出处：《华西口腔医学杂志》2017年01期 　论文类型：期刊论文

【摘要】：目的制备软骨细胞外基质(CECM)取向多孔支架,评价其理化特性及对脂肪干细胞(ADSCs)的相容性。方法切取新鲜猪关节软骨片,匀浆后离心筛选出直径50~500 nm左右的软骨纤维,经Triton X-100脱细胞后制备成浓度6%的CECM浆料,通过定向结晶和冷冻干燥后交联即得CECM取向支架。对CECM取向支架的理化性能和细胞相容性进行评价。结果 CECM取向支架横断面为均匀的多孔网状结构,孔壁上密布软骨纤维,纵断面为垂直管状结构;支架呈苏木精-伊红红染,甲苯胺蓝、番红O、天狼星红染色均呈阳性;支架孔隙率、吸水率和纵向压缩弹性模量分别为95.455%±0.910%、95.889%±1.071%和(40.208±5.097)k Pa;ADSCs在支架上黏附和增殖,并均匀长入支架孔隙内。结论 CECM取向支架的成分与天然软骨相似,生物相容性良好,孔隙结构、孔径大小适于种子细胞黏附、增殖和长入,是一种比较理想的软骨组织工程支架。
[Abstract]:Objective to prepare porous chondrocyte matrix (CECM) oriented scaffolds and evaluate their physicochemical properties and compatibility with adipose stem cells (ADSCs). Methods fresh porcine articular cartilage slices were obtained. The cartilage fibers with diameter of 50 ~ 500nm were selected by centrifugation after homogenization, and the CECM slurry with concentration of 6% was prepared by Triton X-100 acellular. CECM oriented scaffolds were obtained by directional crystallization and freeze-drying crosslinking. The physicochemical properties and cytocompatibility of CECM oriented scaffolds were evaluated. The cross section of CECM oriented scaffold is a uniform porous mesh structure. The wall of the hole is covered with cartilage fibers, and the longitudinal section is a vertical tubular structure. The stents were stained with hematoxylin-iridium, toluidine blue, phannin Oand Sirius red. The porosity, water absorption and longitudinal compression elastic modulus of the scaffold were 95.455% 卤0.910 9% 卤1.071% and 40.208 卤5.097k Pa. respectively. ADSCs adheres and proliferates on the scaffold and grows into the scaffold pore evenly. Conclusion the composition of CECM oriented scaffold is similar to that of natural cartilage with good biocompatibility and pore structure. The pore size is suitable for seed cell adhesion, proliferation and growth. It is an ideal scaffold for cartilage tissue engineering.
【作者单位】：天津医科大学口腔医院正畸科;烟台市口腔医院正畸科;南开大学生命科学学院生物活性材料教育部重点实验室;天津市天津医院脊柱外科;
【基金】：国家自然科学基金(31300798,31470937,81572154) 天津市卫生局科技攻关重点项目(15KG125,16KG114)~~
【分类号】：R318.08;R68
【正文快照】： 3.南开大学生命科学学院生物活性材料教育部重点实验室,天津300071;4.天津市天津医院脊柱外科,天津300211由创伤、炎症、肿瘤等引起的颞下颌关节软骨组织缺损是临床较常见的疾患。由于软骨组织退变呈渐进性发展且自身修复能力极为有限,目前应用于临床的多种治疗方法如手术、软

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