肥胖2型糖尿病患者与大鼠胃旁路术后血清总胆汁酸水平的变化

发布时间：2018-02-01 18:00

本文关键词： 胃旁路术肥胖型糖尿病胆汁酸　出处：《中国病理生理杂志》2017年03期 　论文类型：期刊论文

【摘要】：目的:观察胃旁路术对肥胖2型糖尿病患者及肥胖2型糖尿病模型大鼠血清总胆汁酸水平的影响,并探讨其分子机制。方法:收集并分析2011年6月~2016年6月暨南大学附属第一医院肥胖2型糖尿病患者共87例术后血糖恢复情况及血清总胆汁酸水平变化数据。动物实验选用SD大鼠,高脂喂养联合腹腔注射低剂量链脲佐菌素的方法制作肥胖2型糖尿病模型,分为正常对照(普通饲料喂养非手术)组、胃旁路手术组及假手术组,检测手术前后大鼠血糖及血清总胆汁酸水平,HE染色观察大鼠胰腺组织病理变化,ELISA方法检测大鼠肝组织胆固醇7α-羟化酶(CYP7A1)含量,real-time PCR和Western blot分别检测各组大鼠肝组织CYP7A1和小分子异源二聚体伴侣(SHP)mRNA和蛋白水平。结果:肥胖2型糖尿病患者及模型大鼠胃旁路术后空腹血糖降低,血清总胆汁酸水平升高,肝组织合成胆汁酸经典途径的限速酶CYP7A1含量降低,CYP7A1 mRNA和蛋白表达量均减少,肝组织胆汁酸合成的负性调节因子SHP mRNA和蛋白表达量均增加,与假手术组比较差异显著(P0.05)。结论:胃旁路手术后肥胖2型糖尿病患者及模型动物血清总胆汁酸水平升高,且该变化并非因经典途径合成胆汁酸增多。
[Abstract]:Objective: to observe the effect of gastric bypass on serum total bile acid levels in obese type 2 diabetic patients and obese type 2 diabetic rats. Methods:. From June 2011 to June 2016, 87 patients with type 2 diabetes mellitus who were obese in the first affiliated Hospital of Jinan University were collected and analyzed. The data of blood glucose recovery and serum total bile acid level after operation. Animal experiment selection. SD rats. Methods the obese type 2 diabetic model was established by high fat feeding and intraperitoneal injection of low dose streptozotocin. The model was divided into normal control group, gastric bypass group and sham operation group. The levels of blood glucose and serum total bile acid were detected before and after operation. The pathological changes of pancreas were observed by HE staining. ELISA method was used to detect the content of cholesterol 7 伪 -hydroxylase (CYP7A1) in rat liver tissue. Real-time PCR and Western blot were used to detect CYP7A1 and small molecule heterodimer chaperones in liver tissue of rats in each group. Results: fasting blood glucose decreased after gastric bypass in obese type 2 diabetic patients and model rats. The level of serum total bile acid increased and the expression of CYP7A1 mRNA and protein decreased. The expression of negative regulatory factor SHP mRNA and protein in hepatic bile acid synthesis was increased. Conclusion: the serum total bile acid level of obese type 2 diabetic patients and model animals after gastric bypass surgery is higher than that in sham operation group. This change is not due to the increase of bile acid synthesis by classical pathway.
【作者单位】：暨南大学附属第一医院普通外科;暨南大学附属第一医院内镜中心;暨南大学医学院生理学系;
【基金】：国家自然科学基金资助项目(No.31401001) 广东省自然科学基金资助项目(No.2016A030310086) 广州市科技计划(No.2014Y2-00097) 中央高校基本科研业务费专项资金资助项目(No.21615333) 暨南大学第一临床医学院科研培育专项基金资助项目(No.2013104)
【分类号】：R656.61
【正文快照】： 随着人们饮食结构的变化、生活方式的改变,近年来肥胖2型糖尿病患者逐年增多[1],据统计我国超重或肥胖人口约占总人口的1/4,且超重与肥胖人群2型糖尿病患病率分别为12.8%和18.5%,并且我国2型糖尿病患者中约50%腹型肥胖[2-3]。肥胖2型糖尿病患者药物治疗疗效欠佳[4]。而近年来

【相似文献】