双皮质核素钙调节素依赖激酶-1及Ki-67在胰部分切除术后的表达及分布

发布时间：2018-02-04 16:17

本文关键词： 双皮质核素钙调素依赖性激酶-1 Ki-67 胰大部切除调控机制　出处：《大理学院》2015年硕士论文　论文类型：学位论文

【摘要】：目的:观察大鼠胰大部分切除术后双皮质核素钙调素依赖性激酶-1(DCAMKL-1)和Ki-67在胰组织中的表达及分布,探讨胰岛再生的分子调控机制。方法:35只成年SD大鼠随机分为正常对照组(5只)和模型组(30只)。模型组再分为6个亚组,每组5只,手术切除胰约60%~70%并于术后1 d、2d、3d、4d、5d、6 d分别取残存胰,免疫组织化学PV-9001染色法观察DCAMKL-1和Ki-67在胰组织内的表达及分布。结果:DCAMKL-1和Ki-67在正常胰组织有少量表达,表达主要分布在胰腺导管、胰岛和腺泡之间的间质。模型组术后1 d,主胰管周围可见大量细胞增殖呈蜂巢状样排列,DCAMKL-1和Ki-67表达显著增高,3 d时主导管周围增殖的细胞明显增多并在其周围形成较多的小导管,DCAMKL-1和Ki-67表达于其上皮并达到顶峰,4 d后开始下降,但至6 d时仍高于正常。与正常对照组相比,DCAMKL-1在胰腺腺泡及胰岛内的表达变化不明显,而Ki-67术后5 d时达到高峰,6 d后开始下降。结论:胰部分切除后胰腺再生中,DCAMKL-1主要表达在胰主导管上皮,DCAMKL-1+细胞在主胰管周围增殖并形成新生导管,DCAMKL-1+细胞可能为胰干细胞,DCAMKL-1可能是成体胰腺干细胞的表面标志。
[Abstract]:Objective: to investigate the expression and distribution of Ki-67 and calmodulin-dependent kinase (DCAMKL-1) in rat pancreatic tissue after partial pancreatectomy. Methods 35 adult SD rats were randomly divided into normal control group (n = 5) and model group (n = 30). The model group was subdivided into 6 subgroups with 5 rats in each group. About 60% of the patients underwent surgical resection of the pancreas, and the residual pancreas was removed at 1 day, 2 days, 3 days, 4 days, 5 days and 6 days, respectively. Immunohistochemical PV-9001 staining was used to observe the expression and distribution of DCAMKL-1 and Ki-67 in pancreatic tissues. There was a little expression of DCAMKL-1 and Ki-67 in normal pancreatic tissues. The expression was mainly distributed in the pancreatic duct, the interstitial between the pancreatic islets and the acinus. 1 day after operation, a large number of cells proliferated around the main pancreatic duct in the model group. The expression of DCAMKL-1 and Ki-67 increased significantly at the 3rd day, the number of proliferating cells around the dominant tube increased significantly and more small ducts were formed around them. The expression of DCAMKL-1 and Ki-67 began to decrease 4 days after reaching the peak of the epithelium, but it was still higher than normal at 6 days, compared with the normal control group. The expression of DCAMKL-1 in pancreatic acinar and islets showed no significant change, but reached the peak at 5 d after Ki-67 and began to decrease 6 d after Ki-67. Conclusion: pancreatic regeneration after partial pancreatectomy. DCAMKL-1 was mainly expressed in DCAMKL-1 cells, which proliferated around the main pancreatic duct and formed new ducts. DCAMKL-1 cells may be pancreatic stem cells. DCAMKL-1 may be a surface marker of adult pancreatic stem cells.
【学位授予单位】：大理学院
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R657.5

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