次氯酸消毒液对皮肤软组织感染常见病原体体外杀菌试验效果分析

发布时间：2018-02-13 06:00

本文关键词： 次氯酸皮肤软组织感染病原体　出处：《中华医院感染学杂志》2017年08期 　论文类型：期刊论文

【摘要】：目的探讨次氯酸对皮肤软组织感染常见病原体(包括标准菌株及临床菌株)的体外杀菌效果,为临床应用提供依据。方法根据《消毒技术规范》将0.5麦氏单位的临床分离菌株菌悬液与次氯酸作用1min和10min,然后将作用后的液体与中和剂混合中和消毒效果;普通病原体的混合液取1.0ml进行琼脂倾注法培养,化脓性链球菌和非结核分枝杆菌的混合液取0.1ml分别在血平板和7H10平板上进行涂布法培养;除非结核分枝杆菌培养7d外,其他病原体培养48h后进行菌落计数,计算杀菌对数值。结果次氯酸作用标准菌株1min或10min均可将菌量从0.5麦氏单位(相当于107)降到0;临床菌株包括化脓性链球菌、耐甲氧西林金黄色葡萄球菌(MRSA)、耐碳青霉烯类的鲍氏不动杆菌(CRAB)、耐碳青霉烯类的铜绿假单胞菌(CRPA)、耐碳青霉烯类的肺炎克雷伯杆菌(CRKB)、脓肿分枝杆菌、龟分枝杆菌和产气荚膜杆菌也得到同样结果。结论本试验首次系统全面检测次氯酸对皮肤软组织感染常见的病原体(包括非结核分枝杆菌、厌氧菌、真菌等)均有很好的体外杀菌活性,可以作为感染伤口的局部用药。
[Abstract]:Objective to study the bactericidal effect of hypochloric acid on common pathogens (including standard and clinical strains) of skin and soft tissue infection in vitro. Methods according to the Technical Specification for Disinfection, the suspension of the clinical isolated strain of 0.5 Mak's unit was treated with hypochloric acid for 1 min and 10 min, and then the mixture of neutralizer and neutralizer was used to neutralize the disinfection effect. 1.0 ml mixture of common pathogens was taken for Agar pouring culture, 0.1 ml mixture of Streptococcus suppurans and Mycobacterium tuberculosis was coated on blood plate and 7H10 plate respectively, unless Mycobacterium tuberculosis was cultured for 7 days, Other pathogens were cultured for 48 hours to calculate the bactericidal logarithm. Results the amount of bacteria could be reduced from 0.5 Mak's unit (equivalent to 107) to 0 in 1 minute or 10 min by hypochlorite. Clinical strains included Streptococcus pyogenes. Methicillin-resistant Staphylococcus aureus, carbapenem resistant Acinetobacter baumannii, carbapenem resistant Pseudomonas aeruginosa, Carbicillin-resistant Klebsiella pneumoniae, Mycobacterium abscess. Conclusion for the first time, the common pathogens of hypochloric acid in skin and soft tissue infection (including non-tuberculous mycobacteria, anaerobic bacteria) were detected systematically and comprehensively. Fungi) have good bactericidal activity in vitro and can be used as local drug for infected wounds.
【作者单位】：复旦大学附属中山医院感染管理科;复旦大学附属中山医院临床微生物试验室;
【基金】：复旦大学附属中山医院基金资助项目(2016ZSG-209) 上海市第四轮公共卫生三年行动计划重点学科基金资助项目(15GWZK0101)
【分类号】：R613

【相似文献】