运动对异氟烷处理大鼠成年后物体识别的影响

发布时间：2018-02-20 18:07

本文关键词： 运动生长和发育异氟烷性别因素物体识别　出处：《解放军医学杂志》2017年07期 　论文类型：期刊论文

【摘要】：目的探讨运动对发育期多次异氟烷处理大鼠成年后物体识别能力的影响。方法选取生后7d(P7)的SD大鼠110只,随机分为正常对照组(Naive),运动组(Naive+PE;P21开始,跑台30min/d,5次/周,连续6周),异氟烷组(Iso;P7、P9、P11吸入异氟烷2h,共3次)和异氟烷运动组(Iso+PE)。P67(运动结束即刻)对4组大鼠进行旷场和物体识别行为学测试,P95(运动结束后4周)再次进行物体识别实验,记录大鼠的旷场及新旧物体识别行为学表现。结果运动对各组大鼠成年后旷场试验的影响差异无统计学意义(P0.05)。与Naive相比,P67时Iso组雌性大鼠辨别指数(DR)明显增强,差异有统计学意义(P=0.034),而Iso组雄性大鼠P67和P95时两次测试DR均有增强趋势,但差异无统计学意义(P=0.341、P=0.052)。与非运动组相比,无论是否经过异氟烷处理,运动组雄鼠DR均明显增强(与Naive组及Iso组比较,P67时P=0.050、P=0.017,P95时P=0.037、P=0.019);与Naive组相比,运动组雌鼠P67时DR无明显差异,但到P95时明显增强(P=0.004);与Iso组相比,P67时运动组DR显著下降(P=0.036),而P95时有增强趋势(P=0.095)。与雄鼠相比,P67时非运动组雌鼠DR明显增强(Naive组,P=0.022;Iso组,P=0.011),到P95时,仅没有经过异氟烷处理的两组雌鼠DR高于雄鼠(Naive组,P=0.008;Naive+PE组,P=0.017)。结论发育期多次异氟烷处理对大鼠成年后非空间记忆的物体识别能力无明显损害;发育期接受规律运动能够促进大鼠成年后的物体识别能力,雄鼠的运动获益较大,而雌鼠的运动获益仅体现在远期效果上。
[Abstract]:Objective to investigate the effect of exercise on the ability of object recognition in adult rats treated with isoflurane for several times. Methods 110 SD rats were randomly divided into two groups: the normal control group was naive PEP21, and the running stage was 30 min / d for 5 times a week. For 6 consecutive weeks, isoflurane group (Isopropane P7) P9P11 inhaled isoflurane for 2 h (3 times) and isoflurane exercise group (Iso PEN. P67 (immediately after exercise) were used to conduct open field and object recognition behavioral tests P95 (4 weeks after exercise). Results there was no significant difference in the effect of exercise on the open field test in adult rats. Compared with Naive, the discrimination index of female rats in P67 group was significantly higher than that in P67 group. The difference was statistically significant (P < 0. 034), while in the Iso group, there was an increasing trend in both P67 and P95 Dr, but there was no significant difference between the two groups. Compared with the non-exercise group, the two groups were treated with isoflurane or not. Compared with Naive group and Iso group, the Dr of male rats in exercise group was significantly increased (compared with P67, P67, 0.017, P95, P0. 037, P0. 019, compared with Naive group, there was no significant difference in P67 Dr between exercise group and Naive group. Compared with the Iso group, the Dr of exercise group decreased significantly, while that of P95 showed a tendency to increase. Compared with male rats, female Dr in P67 group increased significantly compared with that in P67 group, and P0. 022 Iso group (P0. 011), P0. 011%, P0. 022 Iso group (P0. 011%) in P67 group, and P0. 095% in P95 group. The Dr of female rats without isoflurane treatment was higher than that of naive group P0. 008 naive PE group (P 0. 017). Conclusion there is no significant damage to the recognition ability of non-spatial memory in adult rats after multiple isoflurane treatments. The regular exercise in the developmental period can promote the ability of object recognition in adult rats, while the male gains more, while the female gains only in the long-term effect.
【作者单位】：解放军总医院第一附属医院麻醉科;锦州医科大学研究生院;解放军总医院第一附属医院全军创伤修复与组织再生重点实验室;解放军总医院第一附属医院骨科;
【基金】：国家自然科学基金(81272030) 吴阶平医学基金会临床科研专项资助基金(320.6750.13220)~~
【分类号】：R614

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