吲哚美辛对大鼠创伤后异位骨化形成中Sclerostin及COX-2表达的影响

发布时间：2018-02-23 16:53

本文关键词： 骨硬化蛋白异位骨化 COX-2 吲哚美辛免疫组化　出处：《遵义医学院》2017年硕士论文　论文类型：学位论文

【摘要】：目的:探讨吲哚美辛对大鼠创伤后异位骨化(Heterotopic ossification,HO)形成中骨硬化蛋白(Sclerostin,SO)及COX-2的表达特点及其对HO形成的影响,为临床诊断、预防和治疗HO提供依据。方法:1.选取30只雄性SPF级SD(Sprague dawley)大鼠行左小腿X线检查并随机抽取5只大鼠抽血行ELISA测量血清SO水平后放回笼中,适应性喂养1周后随机分为实验组10只、模型组10只、对照组10只。无菌条件下,实验组和模型组行左后肢跟腱切断诱导HO模型,对照组仅切开皮肤未切断跟腱。实验组吲哚美辛10mg/(kg·d)浓缩液灌胃4周,模型组及对照组分别给予同等容积生理盐水灌胃。2.造模后第5、第10周,每组摄X线片观察左跟腱部位密度变化及异位骨化评级;每组取5只抽血行ELISA测量血清SO含量;每组处死5只取左侧跟腱行大体形态观察、HE染色及免疫组化检测COX-2表达。数据以均数±标准差((?)±s)表示,应用SPSS16.0统计软件进行统计分析,检验水准α=0.05。结果:1.第5周X线检查结果其中模型组7只、实验组2只出现骨化影,对照组骨化影未现。模型组中COX-2阳性表达,与对照组比较有统计学意义(P0.05);而实验组COX-2表达弱阳性,与模型组比较有统计学意义(P0.05);对照组COX-2呈现阴性或者弱阳性,无统计学意义(P0.05)。模型组SO含量较造模前降低,且均低于对照组及实验组(P0.05);实验组SO浓度升高,与对照组比较有统计学意义(P0.05);对照组血清SO浓度变化无统计学意义(P0.05)。2.第10周X线各组检查结果表明模型组5只、实验组2只出现骨化影;对照组未现骨化影。模型组COX-2表达强阳性,与对照组比较有统计学意义(P0.05);实验组COX-2表达弱阳性,与模型组比较有统计学意义(P0.05);对照组COX-2表达阴性或者弱阳性。模型组血清SO含量较造模前降低,且低于对照组及实验组(P0.05);实验组血清SO浓度升高,与对照组比较有统计学意义(P0.05);对照组血清SO浓度变化无统计学意义(P0.05)。结论:1.HO形成伴随着血清SO水平下调。2.HO形成伴随COX-2表达升高。3.吲哚美辛可抑制HO发生并可同时上调SO及下调COX-2表达。
[Abstract]:Objective: to investigate the effects of indomethacin on the expression of bone sclerosing protein (Sclerostinin so) and its effect on Ho formation in rats with heterotopic ossification (HOO) after trauma. Methods 30 male SPF grade SD(Sprague dawley rats were selected for X-ray examination of left calf, and 5 rats were randomly drawn to measure serum so level by ELISA and put back into cage. After one week of adaptive feeding, the rats were randomly divided into experimental group (n = 10), model group (n = 10) and control group (n = 10). Indomethacin (10 mg / kg 路d) concentrated solution was perfused for 4 weeks in the control group, and the model group and the control group were given the same volume of saline respectively. The rats in the model group were given the same volume of saline for 5 and 10 weeks after the establishment of the model, and the rats in the control group were given the same volume of saline for 4 weeks. The density and ectopic ossification of the left Achilles tendon were observed by X-ray film in each group, and the serum so content was measured by ELISA in 5 rats in each group. In each group, 5 left Achilles tendons were sacrificed for gross morphological observation, HE staining and immunohistochemical analysis of COX-2 expression. 卤s): using SPSS16.0 statistical software for statistical analysis, the test level is 伪 0.05.Results: 1. The results of X-ray examination at the fifth week included 7 rats in the model group, 2 in the experimental group and 2 in the control group. The positive expression of COX-2 was found in the model group, but no ossification shadow was found in the control group. Compared with the control group, the expression of COX-2 in the experimental group was weakly positive, which was significantly higher than that in the model group, while the COX-2 in the control group was negative or weakly positive, and there was no significant difference between the control group and the control group (P 0.05). The content of so in the model group was lower than that in the model group. All of them were lower than those of the control group and the experimental group (P 0.05), the concentration of so in the experimental group was higher than that in the control group (P 0.05), and the serum so concentration in the control group had no statistical significance (P 0.05) .2.The results of X-ray examination in each group at the 10th week showed that there were 5 rats in the model group. Ossification was found in 2 rats in the experimental group, no ossification shadow in the control group. The expression of COX-2 was strongly positive in the model group, which was significantly higher than that in the control group (P 0.05), and the expression of COX-2 was weakly positive in the experimental group. Compared with the model group, the expression of COX-2 in the control group was negative or weakly positive. The serum so content in the model group was lower than that in the model group and lower than that in the control group and the experimental group, and the serum so concentration in the experimental group was higher than that in the control group. Compared with the control group, there was statistically significant difference in serum so concentration between the control group and the control group. Conclusion: 1. Ho formation is accompanied by down-regulation of serum so level. 2.Ho formation accompanied by increased COX-2 expression. Indomethacin can inhibit Ho occurrence and simultaneously. Upregulation of so and down-regulation of COX-2 expression.
【学位授予单位】：遵义医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R681

【相似文献】