人血管基质片段联合脂肪干细胞促进裸鼠放射性皮肤损伤的愈合

发布时间：2018-02-24 09:22

本文关键词： 血管基质片段细胞脂肪干细胞放射性皮肤损伤血管化裸鼠　出处：《山东大学学报(医学版)》2017年09期 　论文类型：期刊论文

【摘要】：目的评价人血管基质片段(SVF)联合脂肪干细胞(ASCs)移植对裸鼠放射性皮肤损伤愈合的影响。方法从健康成年女性脂肪抽吸物酶解获取SVF及ASCs。制备裸鼠放射性皮肤损伤模型后,随机选取SVF+ASCs、SVF、ASCs、对照组的创面给予干预。术后7、14 d观察创面愈合情况;术后第14天取材,行HE染色观察创面炎性反应,免疫组织化学染色计数CD31阳性细胞的分布,Masson染色观察胶原排列情况。结果 SVF+ASCs组14 d时创面愈合完全,对照组创面仍有肉芽组织;ASCs、SVF组创面仍有部分区域未愈合。SVF+ASCs组炎性细胞计数较ASCs组、SVF组及对照组明显减少,微血管计数CD31细胞阳性率明显高于其余3组,Masson染色SVF+ASCs组胶原排列较其他3组整齐。结论 SVF+ASCs联合移植,可促进裸鼠放射性皮肤损伤的愈合,具有良好的应用前景。
[Abstract]:Objective to evaluate the effect of human vascular matrix fragment (SVF) combined with adipose stem cell (ASCs) transplantation on the healing of radiation induced skin injury in nude mice. Methods SVF and ASCs were obtained from healthy adult female fat extracts. The wound of the control group was treated with intervention at random. The wound healing was observed on the 14th day after operation, and the inflammatory reaction of the wound was observed by HE staining on the 14th day after operation. The distribution of CD31 positive cells was counted by immunohistochemical staining. The collagen arrangement was observed by Masson staining. Results the wound healed completely at 14 days in SVF ASCs group. The number of inflammatory cells in ASCs group was significantly lower than that in ASCs group. The positive rate of CD31 cells in microvessel count was significantly higher than that in the other three groups. Conclusion combined transplantation of SVF ASCs can promote the healing of radiation skin injury in nude mice and has a good application prospect.
【作者单位】：中国医学科学院北京协和医学院北京协和医院整形外科协和转化医学中心;清华大学附属北京清华长庚医院整形外科;中国医学科学院北京协和医学院北京协和医院放疗科协和转化医学中心;
【基金】：国家自然科学基金青年基金(81601694)
【分类号】：R622

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