双侧迷走神经切除对大鼠肺缺血再灌注损伤炎症反应的影响

发布时间：2018-02-24 14:05

本文关键词： 再灌注损伤肺损伤炎性反应迷走神经　出处：《解放军医学杂志》2017年07期 　论文类型：期刊论文

【摘要】：目的观察双侧迷走神经切除对大鼠肺缺血再灌注损伤(LIRI)导致的炎症反应的影响。方法 SD大鼠24只,随机分为假手术组(S组)、缺血再灌注组(IR组)和双侧迷走神经切断合并缺血再灌注组(NIR组),每组8只。于缺血前、再灌注0.5h及再灌注4h抽取动脉血进行血气分析,观察动脉血氧分压(Pa O2)及肺泡动脉氧分压差(A-a DO2)的变化。实验结束时取左肺测量肺组织的湿/干重比(W/D),于光学显微镜下观察缺血再灌注后肺的病理学改变,采用ELISA检测肺组织匀浆中肿瘤坏死因子-α(TNF-α)、白细胞介素-10(IL-10)含量及髓过氧化物酶(MPO)活性等炎性指标的水平。结果与S组比较,IR组Pa O2明显降低,A-a DO2明显升高,W/D及肺组织匀浆中TNF-α、IL-10含量和MPO活性明显增加,肺组织炎性细胞浸润、肺泡腔红细胞增多、肺泡破坏及组织水肿明显(P0.05)。与IR组相比,NIR组Pa O2进一步降低,肺组织炎性反应及病理学损伤更加严重(P0.05),但A-a DO2无明显变化。结论切断双侧迷走神经可加重大鼠肺缺血再灌注损伤,提示迷走神经完整性在LIRI的炎性反应调节中具有重要作用。
[Abstract]:Objective to observe the effect of bilateral vagotomy on inflammation induced by lung ischemia-reperfusion injury in rats. The rats were randomly divided into sham operation group (group S) and ischemia reperfusion group (group IR) and bilateral vagotomy combined with ischemia reperfusion group (n = 8). Arterial blood was extracted for blood gas analysis before ischemia, 0.5 h after reperfusion and 4 h after reperfusion. The changes of arterial partial pressure of oxygen (PaO2) and alveolar arterial oxygen pressure difference (A-a DO2) were observed. At the end of the experiment, the left lung was taken to measure the wet / dry weight ratio of lung tissue (W / D), and the pathological changes of the lung after ischemia reperfusion were observed under optical microscope. ELISA was used to detect the contents of tumor necrosis factor- 伪 (TNF- 伪), interleukin-10 (IL-10) and the activity of myeloperoxidase (MPO) in lung homogenate. Results compared with S group, Pao _ 2 in IR group significantly decreased the level of TNF- 伪 -TNF- 伪, interleukin-10 IL-10 and the activity of myeloperoxidase (MPO). Results compared with S group, Pao _ 2 in IR group was significantly lower than that in S group. The content of TNF- 伪 IL-10 and the activity of MPO in the homogenate were significantly increased. Pulmonary inflammatory cells infiltration, alveolar erythrocyte proliferation, alveolar destruction and tissue edema were significantly increased (P0.05). Compared with IR group, Pao _ 2 in NIR group was further decreased. The inflammatory reaction and pathological injury of lung tissue were more serious than that of P0.05, but A-a DO2 had no obvious change. Conclusion bilateral vagotomy can aggravate lung ischemia-reperfusion injury in rats, suggesting that vagus nerve integrity plays an important role in the regulation of inflammatory response of LIRI.
【作者单位】：重庆医科大学附属第一医院麻醉科;四川大学华西医院麻醉科;
【基金】：国家自然科学基金(81170077) 国家卫生部临床重点专科建设项目(财社[2011]170号) 重庆市医学重点学科建设项目(渝卫科教[2007]2号)~~
【分类号】：R614

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