5-氮杂胞苷和地塞米松对大鼠骨髓间充质干细胞分化作用的实验研究

发布时间：2018-02-25 17:20

本文关键词： -氮杂胞苷骨髓间充质干细胞糖皮质激素成骨分化　出处：《中国矫形外科杂志》2017年01期 　论文类型：期刊论文

【摘要】：[目的]探究5-氮杂胞苷和地塞米松对大鼠骨髓间充质干细胞(mesenchymal stem cells,MSCs)成骨分化能力的影响。[方法]SD大鼠骨髓间充质干细胞体外扩增和传代培养,流式细胞术鉴定细胞表面标志,取3代细胞随机分为空白组、激素组、激素+5-氮杂胞苷组,相应药物干预3 d后成骨诱导分化14 d,RT-PCR和Western blot检测Runx2和PPARγ的表达。[结果]所取得骨髓间充质干细胞纯度和均一性好,细胞表面标记CD90高表达,CD34、CD45低表达。第3代细胞经药物干预和成骨诱导后,与空白组相比,激素组Runx2基因表达降低,PPARγ基因升高,5-氮杂胞苷+激素组结果与激素组相反(P0.05)。[结论]激素能抑制骨髓间充质干细胞成骨分化,促进成脂分化,5-氮杂胞苷能干预激素的这种作用,提高骨髓间充质干细胞分化潜能。
[Abstract]:[objective] to investigate the effects of 5-azacytidine and dexamethasone on the osteogenic differentiation of mesenchymal stem cells in rat bone marrow. [methods] Sprague-Dawley rat bone marrow mesenchymal stem cells (BMSCs) were amplified and cultured in vitro, and cell surface markers were identified by flow cytometry. Three passages of cells were randomly divided into blank group, hormone group and hormone 5-azacytidine group. The expression of Runx2 and PPAR 纬 in bone marrow mesenchymal stem cells was detected by RT-PCR and Western blot on the 14th day after the corresponding drug intervention. [results] the purity and homogeneity of bone marrow mesenchymal stem cells were good. The high expression of CD90 on the surface of the cells and the low expression of CD34 / CD45 were observed in the third passage cells after drug intervention and osteogenesis induction, compared with the blank group. The expression of Runx2 纬 gene in hormone group was decreased. The result of 5-azacytidine hormone group was opposite to that of hormone group. [conclusion] hormone can inhibit osteogenic differentiation of bone marrow mesenchymal stem cells and promote adipogenic differentiation of 5-azacytidine intervention hormone. Enhance the differentiation potential of bone marrow mesenchymal stem cells.
【作者单位】：华中科技大学同济医学院附属协和医院骨科;
【基金】：国家自然科学基金资助项目(编号:81271969)
【分类号】：R681.8

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