改良脊髓半横断损伤大鼠模型的建立及评价

发布时间：2018-02-26 07:03

本文关键词： 脊髓损伤大鼠模型改良　出处：《中国医科大学学报》2017年03期 　论文类型：期刊论文

【摘要】：目的建立一种损伤更小、存活率更高、适用范围更广的改良的脊髓半横断损伤大鼠模型。方法将20只雌性SpragueDawley大鼠随机分为常规脊髓半横断模型组和改良脊髓半横断模型组,分别采用常规和改良的脊髓半横断法制备大鼠脊髓损伤模型,分别于术后1 d、3 d、1周和2周通过Basso-Beattie-Bresnahan(BBB)运动功能学评分评价大鼠后肢运动功能的恢复情况,并结合手术时间、术中出血量、术中脊髓后正中线定位以及二次手术显露脊髓的成功率等主要参数,比较2组间的差异。结果改良脊髓半横断模型组较常规脊髓半横断模型组手术时间更短,术中出血量明显减少,BBB运动功能学评分更优,术中脊髓后正中线定位更准确,二次手术显露脊髓的成功率更高。在改良的脊髓半横断模型基础上,成功建立了脊髓瘢痕组织移植模型,成功率可达70%以上。结论改良的脊髓半横断模型在手术时间、术中出血量、运动功能学评分、术中脊髓后正中线定位及二次手术显露脊髓方面均表现出明显优势,更适用于脊髓损伤研究,尤其是非急性期脊髓损伤研究。
[Abstract]:Objective to establish a modified rat model of spinal cord hemitransection injury with less injury, higher survival rate and wider application range. Methods Twenty female SpragueDawley rats were randomly divided into two groups: the normal spinal cord hemitransection model group and the modified spinal cord hemitransection model group. Spinal cord injury models were established by routine and modified hemitransection of spinal cord in rats. The recovery of motor function of hind limbs was evaluated by Basso-Beattie-Bresnahanna BBB score at 1 and 2 days after operation, and combined with operation time. The main parameters, such as intraoperative bleeding, localization of spinal cord posterior median line, and the success rate of second operation, were compared between the two groups. Results the operation time of the modified spinal cord hemitransection model group was shorter than that of the conventional spinal cord hemitransection model group. The intraoperative bleeding volume was significantly reduced and the motor function score of BBB was significantly reduced. The localization of posterior median line of spinal cord was more accurate, and the success rate of exposing spinal cord by secondary operation was higher. Based on the improved model of hemitransection of spinal cord, The successful rate of spinal cord scar transplantation was over 70%. Conclusion the modified spinal cord hemitransection model has the advantages of operation time, intraoperative blood loss, motor function score and so on. The localization of posterior median line of spinal cord and the exposure of spinal cord by secondary operation showed obvious advantages, and were more suitable for the study of spinal cord injury, especially for non-acute spinal cord injury.
【作者单位】：中国医科大学附属第四医院骨科;中国医科大学药学院药物毒理学教研室;中国医科大学附属第一医院骨科;
【基金】：辽宁省自然科学基金(2013021032)
【分类号】：R651.2;R-332

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