带蒂筋膜瓣包裹国产多孔钽修复兔桡骨节段性骨缺损实验研究

发布时间：2018-03-04 02:15

本文选题：多孔钽　切入点：筋膜瓣　出处：《中国修复重建外科杂志》2017年10期 　论文类型：期刊论文

【摘要】：目的观察带蒂筋膜瓣包裹国产多孔钽修复兔桡骨节段性骨缺损的成骨效果。方法选取6~8月龄成年新西兰大白兔60只,雌雄不限,体质量2.5~3.0 kg;制备兔右侧桡骨中段连同骨膜1.5 cm骨缺损模型。实验分为2组,每组30只。实验组于兔右侧前肢切取皮下带蒂筋膜瓣(30 mm×20 mm)包裹多孔钽棒植入桡骨缺损处,对照组于右侧桡骨骨缺损处植入单纯多孔钽棒。于术后当日及4、8、16周行X线片观察,术后4、8、16周取材切片行HE染色及甲苯胺蓝染色观察,术后16周行三点弯曲生物力学测定标本最大载荷力及抗弯曲强度,并行Micro-CT检测及定量分析骨缺损处新生骨体积分数(bone volume fraction,BV/TV),比较两组多孔钽棒体内骨缺损修复能力。结果术后切口均Ⅰ期愈合,无切口感染发生。术后4、8、16周X线片可见两组植入物与宿主骨界面结合越来越牢固,无明显位移,骨折线逐渐消失。HE染色及甲苯胺蓝染色示两组钽-骨界面新生骨组织数量逐渐增多,并沿多孔钽孔隙长入材料内部,骨组织由幼稚到成熟。术后16周,两组三点弯曲试验测定结果示,实验组最大载荷力和抗弯曲强度分别为(96.54±7.21)N、(91.26±1.76)MPa,均明显大于对照组[分别为(82.65±5.65)N、(78.53±1.16)MPa](t=3.715,P=0.004;t=14.801,P=0.000)。Micro-CT扫描观察两组植入物界面、表面均有大量骨组织覆盖,内部不同程度新生骨组织填充;实验组新生骨BV/TV为32.63%±3.56%,显著高于对照组的25.07%±4.34%(t=3.299,P=0.008)。结论带蒂筋膜瓣包裹国产多孔钽可增加局部血运,增强材料骨传导能力,促进节段性骨缺损修复。
[Abstract]:Objective to observe the osteogenic effect of porous tantalum coated with pedicled fascia flap on rabbit radial segmental bone defect. Methods 60 adult New Zealand white rabbits aged 6 ~ 8 months, male and female, were selected. The animal model of bone defect in the middle segment of the right radius with 1.5 cm periosteum was established. The experimental group was divided into two groups, 30 in each group. The experimental group was divided into two groups. The experimental group was divided into two groups. The experimental group was divided into two groups. The experimental group was divided into two groups. The experimental group was divided into two groups. The experimental group was divided into two groups. In the control group, pure porous tantalum rods were implanted at the defect of the right radius. X-ray films were observed on the day after operation and at 816 weeks after operation, and sections were taken from the sections at 816 weeks after operation for HE staining and toluidine blue staining. The maximum load force and bending strength of the specimen were measured by three point bending biomechanics 16 weeks after operation. Bone volume fraction in bone defect was detected and quantitatively analyzed by Micro-CT. The repair ability of bone defect was compared between the two groups. Results all the incisions were healed in the first stage after operation. No incision infection occurred. X-ray films showed that the interface between the implants and the host bone was more and more firm, and there was no obvious displacement between the implants and the host bone. The results of HE staining and toluidine blue staining showed that the number of new bone tissue in tantalum bone interface increased gradually, and the bone tissue grew into the material along the porous tantalum pore, and the bone tissue grew from infantile to mature 16 weeks after operation. The results of three-point bending test in the two groups showed that the maximum load force and bending strength of the experimental group were 96.54 卤7.21 Nou 91.26 卤1.76 mpa, respectively, which were significantly higher than that of the control group [78.53 卤1.16 MPA] of the two groups. The BV/TV of nascent bone in the experimental group was 32.63% 卤3.56, which was significantly higher than that in the control group (25.07% 卤4.34). Conclusion the porous tantalum coated with pedicled fascia flap can increase local blood flow, enhance bone conduction ability and promote segmental bone defect repair.
【作者单位】：华北理工大学附属唐山骨科医院手外科;华北理工大学基础医学院人体解剖系;华北理工大学附属唐山骨科医院关节科;华北理工大学附属医院骨科;华北理工大学医学实验研究中心;
【基金】：国家科技部科技支撑课题资助项目(2012BAE06B03) 河北省科技支撑课题资助项目(16277776D) 河北省医学科学研究重点课题计划资助项目(20160225)~~
【分类号】：R687.3

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