不同浓度一氧化碳释放分子-3对大鼠骨髓间充质干细胞成骨分化的影响

发布时间：2018-03-04 07:08

本文选题：一氧化碳释放分子-　切入点：骨髓间充质干细胞　出处：《山东大学学报(医学版)》2017年07期 　论文类型：期刊论文

【摘要】：目的探讨不同浓度一氧化碳释放分子-3(CORM-3)对大鼠骨髓间充质干细胞(BMSCs)成骨分化的影响。方法实验分A、B两部分,各部分均包括5组,A部分:矿化组,100、200、400μmol/L CORM-3组,对照组;B部分:矿化组,100、200、400μmol/L CORM-3-矿化组,对照组。采用Real-time PCR法和Western blotting法分别检测成骨相关基因Runx2和OPNmRNA与蛋白的表达,茜素红染色检测成骨能力。采用SPSS 19.0软件对数据进行统计学分析。结果 100、200μmol/L CORM-3可促进BMSCs增殖,差异有统计学意义(P=0.034,P0.001);单纯CORM-3对BMSCs成骨分化无影响;BMSCs经200、400μmol/L CORM-3预处理后再行矿化诱导,7 d后大鼠BM SCs的Runx2和OPNmRNA表达增强,与矿化组比较差异有统计学意义(Runx2:P=0.005,P=0.006;OPN:P=0.010,P=0.028),其蛋白表达及21 d后的成骨能力较矿化组均明显增强,且200μmol/L CORM-3预处理的效果优于400μmol/L CORM-3。结论 CORM-3预处理可促进体外BMSCs成骨分化,200μmol/L为CORM-3用于实验的适宜浓度。
[Abstract]:Objective to investigate the effects of different concentrations of carbon monoxide release molecule -3CorM-3 on osteogenic differentiation of rat bone marrow mesenchymal stem cells (BMSCs). Part B of the control group: the mineralized group was treated with 400 渭 mol/L CorM-3- mineralization group and the control group. The expression of Runx2, OPNmRNA and protein were detected by Real-time PCR and Western blotting, respectively. Alizarin red staining was used to detect the osteogenic ability. SPSS 19.0 software was used to analyze the data. Results 100,200 渭 mol/L CORM-3 could promote the proliferation of BMSCs. The difference was statistically significant (P < 0.05), but CORM-3 alone had no effect on the osteogenic differentiation of BMSCs. After pretreatment with 200,400 渭 mol/L CORM-3, the expression of Runx2 and OPNmRNA in BMSCs of rats was enhanced after 7 days of mineralization induction. Compared with the mineralized group, the difference was statistically significant (Runx2: P0. 005: P0. 006) OPN: P0. 010 and P0. 028. The protein expression and the osteogenic ability of the mineralized group were significantly increased after 21 days compared with the mineralized group. The pretreatment effect of 200 渭 mol/L CORM-3 was better than that of 400 渭 mol/L Corm 3. Conclusion CORM-3 pretreatment can promote the osteogenic differentiation of BMSCs in vitro with 200 渭 mol/L as the suitable concentration of CORM-3.
【作者单位】：山东大学口腔医院黏膜病科山东省口腔组织再生重点实验室;青岛市市立医院口腔科;济南市口腔医院儿童牙病科;山东大学口腔医院特诊科山东省口腔组织再生重点实验室;
【基金】：山东省自然科学基金(ZR2015HM019) 山东省教育奖补专项基金(鲁财教指[2014]94号) 济南市高校科技创新计划项目(201401259)
【分类号】：R68

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