高压氧对模型大鼠颅脑损伤CAT、SOD、GSH-Px和Nrf2影响的研究

发布时间：2018-03-05 14:29

本文选题：高压氧　切入点：颅脑损伤　出处：《神经解剖学杂志》2017年03期 　论文类型：期刊论文

【摘要】：目的:研究高压氧对模型大鼠颅脑损伤过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和核因子E2相关因子2(Nrf2)的影响。方法:健康SD大鼠30只,体重250~300 g,4~5月龄,随机分为3组:假手术组、模型组和高压氧组,每组10只,参照Feeney自由落体冲击造模法建立颅脑损伤大鼠模型。假手术组只给予手术,不造成颅脑损伤;模型组和高压氧组给予颅脑损伤处理;高压氧组用高压氧治疗,1次/d,10 d。用ELISA法检测脑组织CAT、SOD、GSH-Px和Nrf2含量,采用实时荧光定量PCR检测脑组织CAT、SOD、GSH-Px和Nrf2 mRNA相对表达量,用Western Blot测定脑组织Nrf2胞质蛋白和Nrf2核蛋白。结果:假手术组、模型组、高压氧组之间的CAT、SOD、GSH-Px、Nrf2 mRNA水平均有差异(P0.05)。模型组脑组织CAT、SOD、GSHPx、Nrf2 mRNA水平低于假手术组(P0.05),而高压氧组则高于模型组(P0.05)。假手术组、模型组、高压氧组之间的脑组织CAT、SOD、GSH-Px和Nrf2核蛋白水平存在差异(P0.05),模型组低于假手术组(P0.05),高压氧组高于模型组(P0.05)。Western Blot检测显示:与假手术组比较,模型组和高压氧组脑组织细胞质(核)中Nrf2蛋白表达量有明显升高(P0.05),高压氧组升高更明显(P0.05)。结论:高压氧对模型大鼠颅脑损伤CAT、SOD、GSH-Px和Nrf2表达具有调节作用,通过这些调节改善机体氧化性损伤。
[Abstract]:Objective: to study the effects of hyperbaric oxygen on catalase catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and nuclear factor E2 related factor (Nrf2) in model rats with craniocerebral injury. The rats were randomly divided into three groups: sham operation group, model group and hyperbaric oxygen group, each group with 10 rats. The model of craniocerebral injury was established by Feeney free falling body impact method. Model group and hyperbaric oxygen group were treated with craniocerebral injury, hyperbaric oxygen group was treated with hyperbaric oxygen for 10 days. The contents of GSH-Px and Nrf2 in brain tissue were measured by ELISA method, and the relative expressions of GSH-Px and Nrf2 mRNA in brain tissue were detected by real-time fluorescence quantitative PCR. Nrf2 cytoplasmic protein and Nrf2 nuclear protein in brain tissue were measured by Western Blot. Results: sham operation group, model group, model group, The level of GSH-PxNrf2 mRNA in brain tissue of model group was lower than that of sham operation group (P 0.05), but that of hyperbaric oxygen group was higher than that of model group. There were significant differences in the levels of GSH-Px and Nrf2 in brain tissue between hyperbaric oxygen group (HBO) group and sham operation group (P 0.05). The results of Western Blot analysis of hyperbaric oxygen group were as follows: compared with sham-operation group, hyperbaric oxygen group was higher than model group (P 0.05). In the model group and hyperbaric oxygen group, the expression of Nrf2 protein was significantly increased in the cytoplasm (nucleus) of brain tissue, especially in the hyperbaric oxygen group. Conclusion: hyperbaric oxygen can regulate the expression of GSH-Px and Nrf2 in CATSODX after craniocerebral injury in the model rats, and the expression of GSH-Px in the hyperbaric oxygen group is even higher than that in the hyperbaric oxygen group. These adjustments improve the oxidative damage of the body.
【作者单位】：庆阳市人民医院神经内科;陕西省第四人民医院急诊科;第四军医大学口腔医院麻醉科;
【基金】：国家自然科学基金(81471265) 陕西省科技统筹创新工程计划项目(2012KTCG01-02)
【分类号】：R-332;R651.15

【相似文献】