Gln增加T2DM大鼠骨骼肌质量的探究

发布时间：2018-03-06 17:11

本文选题：Gln　切入点：2型糖尿病　出处：《昆明理工大学》2017年硕士论文　论文类型：学位论文

【摘要】：肌肉减少症是一种老年多发病。肌肉减少症具有骨骼肌体积减少,肌力下降和生活质量下降3个特点。肌肉减少症的发病原因与年龄、营养、运动、疾病等多种因素相关。肌肉减少症发病的机制是复杂的。糖尿病是一种很常见的老年代谢性疾病,患者因胰岛素的水平下降或胰岛素抵抗导致血糖升高而易患上肌肉减少症。谷氨酰胺(Glutamine,Gln)是生物体的一种非必需氨基酸。由于Gln在生物体中发挥极其重要的作用,又被称为“条件必需氨基酸”。Gln在临床上有多种用途。胰岛素信号通路是生物体调节血糖水平以及促进生物体自身生长的重要信号通路。其中,PI3K/Akt信号通路是胰岛素信号通路下游之一。PI3K/Akt信号通路的下游靶基因成肌分化抗原基因(Myogenic Differentiation Antigen,MyoD)和肌肉环指1基因(muscle RING finger 1,MuRF1)与骨骼肌质量的变化有关。本研究利用高脂饮食结合小剂量链脲佐菌素注射诱导2型糖尿病大鼠模型,分四周、八周进行Gln灌胃治疗。研究Gln对各组(正常对照组、2型糖尿病模型对照组、Gln低剂量治疗组、Gln高剂量治疗组)大鼠MyoD、MuRF1、表达量的影响。探究Gln增加2型糖尿病大鼠骨骼肌质量的分子机制,为Gln用于治疗由2型糖尿病引发的肌肉减少症提供理论依据。本研究还探究了 Gln治疗后在所检测的血液指标中是否存在与2型糖尿病大鼠骨骼肌质量增加可能有关的血液指标。本研究发现,Gln具有增加2型糖尿病大鼠骨骼肌质量的作用。Gln可以分别上调2型糖尿病大鼠MyoD基因和下调MuRF1基因的表达,起到增加骨骼肌质量的作用。Gln治疗后,在所检测的血液指标中不存在与2型糖尿病大鼠骨骼肌质量增加可能有关的血液指标。
[Abstract]:Myopenia is a common disease in the elderly. It has three characteristics: the reduction of skeletal muscle volume, the decrease of muscle strength and the decline of quality of life. The cause of myopenia is associated with age, nutrition and exercise. Diseases and other factors are related. The pathogenesis of myopenia is complex. Diabetes is a very common metabolic disease in the elderly. Patients are prone to myopenia due to decreased insulin levels or increased blood glucose caused by insulin resistance. Glutamine glutamine glutamine is a non-essential amino acid in organisms. Because Gln plays an extremely important role in organisms, It is also called "conditioned essential amino acid". GLN has many clinical uses. Insulin signaling pathway is an important signal pathway for organism to regulate blood glucose level and promote organism's own growth. Among them, PI3K / Akt signaling pathway is insulin. The downstream target gene of PI3K / Akt signaling pathway, Myogenic Differentiation antigenation antigen gene (Myogenic Differentiation antigenia MyoD1) and muscle ring finger 1 gene, muscle RING finger 1 (MuRF1), are related to the changes of skeletal muscle quality. In this study, a high-fat diet combined with low-dose streptourea was used to study the relationship between myogenic differentiation antigen gene and muscle RING finger 1MuRF1 gene in PI3K / Akt signaling pathway. The model of type 2 diabetes mellitus was induced by injection of Zocmectin. In four weeks, To study the effect of Gln on the expression of MyoDnMuRF1 in rats of low dose of Gln in normal control group (normal control group) and low dose of GLN treatment group. To explore the increase of Gln in bone of type 2 diabetic rats by intragastric administration of Gln for 8 weeks, and to explore the effect of Gln on the expression of MyoDX MuRF1 in rats with type 2 diabetes mellitus. The molecular mechanism of the mass of the iliac muscle, To provide the theoretical basis for the use of Gln in the treatment of myopenia caused by type 2 diabetes mellitus. This study also explored whether there is a possible increase in skeletal muscle mass of type 2 diabetic rats in the blood parameters detected after Gln treatment. In this study, we found that GLN can increase skeletal muscle mass in type 2 diabetic rats. GLN can up-regulate MyoD gene and down-regulate MuRF1 gene expression in type 2 diabetic rats, respectively. After treatment with GLN, there were no possible blood indexes related to the increase of skeletal muscle mass in type 2 diabetic rats.
【学位授予单位】：昆明理工大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R685;R587.2

【相似文献】