游离脂肪酸对小鼠骨骼肌干细胞分化及功能的影响

发布时间：2018-03-07 21:42

本文选题：棕榈酸　切入点：骨骼肌干细胞　出处：《南京医科大学学报(自然科学版)》2017年05期 　论文类型：期刊论文

【摘要】：目的 :观察棕榈酸(palmitic acid,PA)对小鼠骨骼肌干细胞分化及功能的影响,为治疗衰老相关的肌少症寻找新思路。方法:对小鼠骨骼肌干细胞诱导分化后,同时予PA(100μmol/L)干预,用荧光染色法、荧光定量PCR测定成肌基因肌球蛋白重链(myosin heavy chains,MyHC)表达;用seahorse能量代谢仪检测细胞的氧耗增加率,荧光定量PCR检测线粒体融合基因线粒体融合素基因1(mitofusin1,Mfn1)、视神经萎缩症蛋白基因(opticatrophy 1,Opa1)和裂解基因发动相关因子基因(dynamin 1-like,Drp1)、线粒体分裂素基因1(fission 1,Fis1)的相对表达量。结果 :PA刺激后,MyHC荧光表达量下降,肌小管融合率减低,同时seahorse能量代谢仪检测细胞的氧耗增加率(oxygen consumption rate,OCR)降低(P0.05);线粒体融合基因无明显异常,但裂解基因表达增加,融合/裂解比值下降(P0.05)。结论:游离脂肪酸抑制骨骼肌干细胞分化、影响线粒体氧耗功能,这可能是通过影响线粒体Drp1基因,增加线粒体裂解实现的。
[Abstract]:Objective: To observe the effect of palmitic acid (palmitic, acid, PA) on mice skeletal muscle stem cell differentiation and function, for the treatment of age-related sarcopenia in search of new ideas. Methods: mouse skeletal muscle stem cells after differentiation, and treated with PA (100 mol/L) intervention, using fluorescence staining, fluorescence quantitative determination of PCR muscle myosin heavy chain gene (myosin heavy chains, MyHC) expression; energy metabolism of seahorse cells was assessed with the increase in oxygen consumption rate, fluorescence quantitative PCR detection of mitochondrial fusion gene mitofusin 1 genes (mitofusin1, Mfn1), optic nerve atrophy protein gene (opticatrophy 1, Opa1) and a lytic gene related genes (dynamin, 1-like, Drp1) mitochondrial fission gene 1 (fission 1, Fis1). Results: the relative expression of PA after stimulation with MyHC fluorescence expression decreased, myotubule fusion rate reduce, and energy metabolism of seahorse detected by fine Cellular oxygen consumption rate of increase (oxygen consumption rate, OCR) decreased (P0.05); mitochondrial fusion gene had no obvious abnormalities, but lytic gene expression increased, fusion / cracking ratio decreased (P0.05). Conclusion: free fatty acids inhibit skeletal muscle stem cell differentiation, affecting mitochondrial oxygen consumption function, which may be affected by mitochondria Drp1 gene, increased mitochondrial fission is achieved.

【作者单位】：南京医科大学第一附属医院老年医学科;
【基金】：国家自然科学基金面上项目(81370950)
【分类号】：R685

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