右美托咪定联合缺血预处理对大鼠肝缺血再灌注损伤的作用

发布时间：2018-03-08 00:09

本文选题：右美托咪定　切入点：缺血预处理　出处：《石河子大学》2015年硕士论文　论文类型：学位论文

【摘要】：目的:探讨右美托咪定联合缺血预处理对大鼠肝缺血再灌注损伤的作用及可能机制。方法:60只健康雄性清洁型SD大鼠,体重(251±18)g,按随机数字表法分为五组(n=12):假手术组(S组)、缺血再灌注组(IR组)、右美托咪定预处理组(Dex组)、缺血预处理组(IP组)和右美托咪定联合缺血预处理组(Dex+IP组)。采用Pringle法分别建立大鼠肝脏缺血再灌注模型,测定肝脏缺血30min再灌注6h后血清中ALT、AST、LDH、TNF-α的浓度。取肝脏组织,通过HE染色观察其病理学改变,TUNEL检测肝脏组织中细胞凋亡数量,免疫组化测定肝脏组织血红素氧合酶-1(heme oxygenase-1,HO-1)的表达。结果:与S组相比,其余各组血清中ALT、AST、LDH及TNF-α浓度明显增高(P0.01);与IR组相比,Dex、IP及Dex+IP组明显降低(P0.01);Dex+IP组明显低于Dex组及IP组(P0.01)。ALT、AST及TNF-α浓度在Dex组与IP组之间无明显差异(P0.05),LDH浓度在Dex组明显低于IP组(P0.01)。肝组织病理学评分IR、Dex、IP及Dex+IP组明显高于S组(P0.01),Dex、IP及Dex+IP组明显低于IR组(P0.01),Dex+IP组明显低于Dex、IP组(P0.01),Dex组与IP组之间未见明显差异(P0.05)。肝细胞凋亡指数IR、Dex、IP及Dex+IP组明显高于S组(P0.01),Dex、IP及Dex+IP组明显低于IR组(P0.01),Dex+IP组明显低于Dex、IP组(P0.01),Dex组与IP组之间未见明显差异(P0.05)。HO-1评分,与S组相比,IR组增高(P0.05),Dex、IP及Dex+IP组明显增高(P0.01);与IR组相比,Dex及IP组增高(P0.05),Dex+IP组明显增高(P0.01);Dex+IP组高于Dex及IP组(P0.05);Dex与IP组之间未见明显差异(P0.05)。结论:右美托咪定及缺血预处理对大鼠肝缺血再灌注损伤均有保护作用,两者联合应用效果更好,其作用均与诱导HO-1的表达有一定关系。
[Abstract]:Objective: to investigate the effect and possible mechanism of dexmetomidine combined with ischemic preconditioning on hepatic ischemia-reperfusion injury in rats. The body weight was 251 卤18g. According to the random number table, the rats were divided into five groups: sham operation group (S group), ischemia reperfusion group (IR group), dexmetomidine preconditioning group (Dex group), ischemic preconditioning group (IP group) and dexmetomidine combined with ischemic preconditioning group (Dex IP group). Rat liver ischemia-reperfusion models were established by Pringle method. The concentration of ALT ASTX LDH TNF- 伪 in serum was measured after 30 min of hepatic ischemia and reperfusion for 6 h. The pathological changes of liver tissue were observed by HE staining and Tunel was used to detect the number of apoptosis in liver tissue. Immunohistochemistry was used to determine the expression of heme oxygenase-1 (HO-1) in liver tissue. Results: compared with group S, the expression of heme oxygenase-1 (HO-1) was detected by immunohistochemistry. The levels of LDH and TNF- 伪 in serum of other groups were significantly higher than those of IR group (P 0.01), compared with IR group, the levels of TNF- 伪 and LDH in Dex group were significantly lower than those in Dex group and IP group. There was no significant difference between Dex group and IP group in the level of LDH and TNF- 伪. The level of LDH in Dex group was significantly lower than that in Dex group and IP group, and the level of TNF- 伪 in Dex group was significantly lower than that in Dex group and IP group (P < 0.05), and there was no significant difference between Dex group and IP group in the concentration of LDH and TNF- 伪. The hepatic histopathology scores in IP group were significantly higher than those in S group (P 0.01) and Dex IP group (P 0.01). There was no significant difference in hepatic histopathology between IP group and IP group. There was no significant difference in hepatocyte apoptosis index between Dex IP group and Dex IP group. It was significantly higher than that in group S (P 0.01) and group Dex IP (P 0.01). There was no significant difference between the two groups (P 0.05). HO-1 score was significantly lower in Dex IP group than that in P0.01DX IP group (P 0.01) and IP group (P < 0.05), and there was no significant difference between the two groups in the scores of P0.05 and HO-1, and there was no significant difference between the two groups. Compared with S group, the levels of P0.05Dexus IP and Dex IP were significantly higher in IR group than those in IR group, and the levels of P0.05 + Dexus IP and Dex IP were significantly higher than those in Dex and IP group. Conclusion: there is no significant difference between P0.05Dex and IP group. Conclusion: there is no significant difference between P0.05Dex group and IP group. Conclusion: compared with IR group and IP group, there is no significant difference between P0.05Dex group and IP group. Conclusion: there is no significant difference between the two groups. Conclusion: there is no significant difference between P0.05Dex group and IP group. Preconditioning had protective effects on hepatic ischemia-reperfusion injury in rats. The effect of the combined treatment was better, and the effect was related to the induction of HO-1 expression.
【学位授予单位】：石河子大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R614

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