钛离子在钛颗粒诱导的假体周围炎症中的作用观察研究

发布时间：2018-03-09 06:30

本文选题：钛离子　切入点：钛颗粒　出处：《南昌大学》2015年硕士论文　论文类型：学位论文

【摘要】：目的:构建小鼠air-pouch植骨气囊模型,并选择钛颗粒所诱导的骨溶解模型,观察在气囊内部注射钛离子溶液或者钛颗粒混悬液后对钛颗粒所诱导的无菌性炎症的程度是否具有一致性,探讨钛离子在炎性因子介导的无菌性松动致炎性过程中其具有的可能作用。方法:雄性昆明近交系小鼠60只,平均约8-10周龄大小,每只体重约为30g左右。20只小鼠作为气囊植骨模型颅骨供体。取余下的40只小鼠按照随机法分为4组,每组10只。分别设定为空白对照组(A组)、钛颗粒组(B组)、rh-VEGF组(C组)和钛离子组(D组)。小鼠背部皮肤备皮后皮下注入无菌空气3m L作为初始气囊,隔日在气囊内加注1ml无菌空气维持,第8天无菌条件下切开气囊并在内壁植入同源颅骨片,完成植骨气囊模型。植骨后第一天在B、C组小鼠气囊中注入0.5m L钛颗粒悬液,D组注入0.5ml钛离子溶液,A组注入0.5m L生理盐水溶液。气囊植骨前第6、7天及植骨后隔天,在C组小鼠气囊内注射0.2m L的rh-VEGF混悬液,A、B、D组则相应注射0.2m L的无菌生理盐水。植骨2周后使用颈椎脱臼法处死小鼠并取囊壁以及骨片行HE染色观察其病理学改变,免疫组化及q RT-PCR检测炎性因子TNF-α、IL-1β、IL-6、VEGF的表达量改变。结果:所有小鼠均存活直至实验完成。大体观察发现A组气囊红肿轻,囊壁上新生血管少见。B、C、D组气囊红肿明显加重,可见较多渗出及新生血管,其中C组最重,B组次之,D组介于B、C组之间。组织学及分子生物学检测表明,B组囊壁炎性反应及骨溶解反应明显,囊壁厚度、细胞密度及TN F-α、IL-1β、IL-6、VEGF表达量较A组对照组明显增加(P0.05);C组囊壁炎性反应及骨溶解最为明显,上述观察指标均高于B组颗粒组(P0.05);D组囊壁亦可见明显炎性反应及轻微骨溶解,但炎性因子的表达相较于B组而言则表达量相当或略低(P0.05),较A组则明显增高(P0.05)。结论:钛离子在体内可以诱导炎性因子的表达,并且同样能够促进VEGF的表达及分泌,效果同加入VEGF促进剂后相当。抑制钛离子释放能够抑制钛颗粒所诱导的无菌性炎性反应。
[Abstract]:Objective: to construct the air-pouch mice bone bag model, and select the model of titanium particle induced osteolysis, is observed in the internal air injection of titanium ion solution or titanium particle suspension on titanium particle induced by the inflammatory degree of consistency, to investigate the titanium ion in aseptic loosening of inflammatory cytokines mediated inflammatory process with its possible role. Methods: Kunming male inbred mice 60, an average of about 8-10 weeks of age size, each weighing about 30g.20 mice as a model of skull bone graft balloon donor. The remaining 40 mice randomly divided into 4 groups, 10 rats in each group. Were set as blank control group (A group), titanium particle group (B group), rh-VEGF group (group C) and titanium ion group (D group). The skin of mice skin after subcutaneous injection of sterile air 3M L as the initial balloon, every other day in the airbag with 1ml sterile air to maintain the 8 days under sterile conditions, cut and skull piece in the inner balloon homologous bone graft implantation, complete the airbag model. The first day after bone grafting in B, C group were injected 0.5m L titanium particles air suspension, D group injected 0.5ml titanium ion solution, the A 0.5m group were injected with L saline solution. The bone grafting before balloon day 6,7 and bone graft in C group were injected 0.2m L rh-VEGF air suspension, A, B, D 0.2m L group was injected with sterile saline. Bone graft 2 weeks after mice were killed by cervical dislocation and cystic wall and bone slices stained with HE to observe its change pathological, immunohistochemical and Q RT-PCR detection of inflammatory factor TNF- alpha, IL-1 beta, IL-6, the expression of VEGF. Results: all the mice survived until the end of the experiment. The general observation group A bag red light, the cyst wall neovascularization rare.B, C, D and airbags significantly heavier, more visible exudation and neovascularization, which The most severe in C group, B group, D group is B, C between the groups. Show that the histological and molecular biological detection, B group of cystic wall inflammation and osteolysis, wall thickness, cell density and TN F- alpha, IL-1 beta, IL-6, VEGF expression increased significantly in A group (P0.05); C group of cystic wall inflammation and osteolysis is most obvious, the observation indexes were higher than that of group B particles (P0.05); group D group also showed cystic wall inflammation and mild osteolysis, but the expression of inflammatory cytokines compared with B group, the expression of P0.05 (equivalent to or slightly lower), was significantly higher than A group (P0.05). Conclusion: the expression of titanium ions can induce inflammatory cytokines in vivo, and also can promote the expression of VEGF and secretion, the effect is the same as adding VEGF accelerator after aseptic inflammatory reaction. Inhibition of titanium ions can inhibit the release of titanium particles induced.

【学位授予单位】：南昌大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R687.4

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