黏着斑激酶在骨肉瘤中的表达及临床意义

发布时间：2018-03-09 10:37

  本文选题：骨肉瘤　切入点：黏着斑激酶　出处：《中国肿瘤临床》2017年10期 　论文类型：期刊论文

【摘要】：目的:探讨骨肉瘤组织中黏着斑激酶(focal adhesion kinase,FAK)的表达情况及其与临床病理特征和预后的关系。方法:收集1999年12月至2011年l2月东南大学附属中大医院和南京军区南京总医院收治的经手术病理确诊的骨肉瘤标本113例,免疫组织化学检测病灶中FAK表达量及磷酸化水平,并通过RNA干扰观察改变FAK的表达与激活水平对骨肉瘤细胞增殖、凋亡、迁移及侵袭活动的影响。结果:70例标本(61.95%)FAK过表达,其中42例(37.17%)p FAK阳性。FAK表达谱与性别、年龄、AJCCⅡA/ⅡB期、病灶部位、术式、术前化疗效果等临床病理参数均无显著相关性。生存分析显示FAK的过表达及其磷酸化显著缩短了总生存时间(overall survival,OS)和无转移生存时间(metastasis-free survival,m FS),其与术前化疗效果是预测骨肉瘤生存时间和转移早晚的两个独立预后因素。细胞学实验则显示FAK的表达和激活可以促进骨肉瘤细胞的增殖、迁移及侵袭活动,并能抑制凋亡。结论:FAK的过表达及其磷酸化与骨肉瘤恶性程度密切相关,可为生存期及预后判断提供一定参考。
[Abstract]:Objective: to investigate the expression of focal adhesion kinase (FAK) in osteosarcoma and its relationship with clinicopathological features and prognosis. Methods: from December 1999 to 2011, the hospital affiliated to Southeast University and the south of Nanjing military region were collected. 113 cases of osteosarcoma confirmed by operation and pathology were treated in Beijing General Hospital. The expression and phosphorylation of FAK were detected by immunohistochemistry, and the effects of FAK expression and activation on the proliferation, apoptosis, migration and invasion of osteosarcoma cells were observed by RNA interference. Among them, 42 cases (37. 17%) showed positive. FAK expression profile and sex, age, AJCC 鈪，

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