骨关节炎患者软骨细胞Fas基因增强子区甲基化水平与Fas蛋白表达的相关性

发布时间：2018-03-10 11:53

本文选题：骨关节炎　切入点：Fas增强子　出处：《中国老年学杂志》2017年13期 　论文类型：期刊论文

【摘要】：目的通过观察骨关节炎(OA)软骨细胞中Fas基因甲基化水平与Fas蛋白表达的相关性,探讨Fas基因增强子区异常甲基化在OA发生和发展中的作用。方法采用亚硫酸氢钠方法处理基因组DNA,甲基化特异性PCR技术和免疫组织化学法分别检测14例健康人和35例OA患者软骨细胞中Fas基因增强子区甲基化水平和相关蛋白表达情况。结果 OA组Fas基因增强子区低甲基化率及软骨细胞Fas蛋白表达阳性率均明显高于健康对照组(P0.01),且Fas基因低甲基化及蛋白表达与性别,年龄无明显相关性(P0.05)。Fas增强子低甲基化与Fas蛋白表达呈显著正相关(P0.01)。结论 OA患者关节软骨细胞Fas基因增强子区呈低甲基化状态,通过上调Fas蛋白表达参与OA的发生发展。
[Abstract]:Objective To observe the effect of osteoarthritis (OA) correlation between methylation of Fas gene in chondrocytes and the level of expression of Fas protein, investigate the hypermethylation of Fas gene promoter in the occurrence and development of OA in vitro. Methods using Sodium Bisulfite processing method of genomic DNA methylation specific PCR technique and immunohistochemical method were used to detect the level of methyl and related proteins in 14 healthy people and 35 patients with OA in chondrocytes of Fas gene promoter expression. Results in the OA group, Fas gene promoter methylation rate of cartilage cells and low Fas protein expression positive rate were significantly higher than the healthy control group (P0.01), and Fas gene methylation and protein expression and gender there was no significant correlation between age, (P0.05).Fas enhancer hypomethylation and Fas protein expression were positively correlated (P0.01). Conclusion OA patients with articular cartilage cells of Fas gene promoter methylation was low - It participates in the development of OA by up-regulating the expression of Fas protein.

【作者单位】：中南大学湘雅二医院骨科;湖南中医药大学医学院生物化学与分子生物学教研室;湖南中医药大学干细胞调控与应用实验室;
【分类号】：R684.3

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