芬太尼诱导的大鼠急性痛觉过敏和脊髓炎性反应有关

发布时间：2018-03-13 16:35

本文选题：芬太尼　切入点：痛觉过敏　出处：《中山大学学报(医学科学版)》2017年01期 　论文类型：期刊论文

【摘要】：【目的】探索芬太尼诱导的急性痛觉过敏模型中,大鼠脊髓促炎因子表达的变化。【方法】64只雄性SD大鼠分为2组(n=32),实验组(Fentanyl组)皮下注射芬太尼60μg/Kg共4次,每次间隔15 min,对照组(Normal saline组)皮下注射生理盐水4次。于注射前1 d(D-1)、注射后1、2、3、4 h及1~5 d对各组大鼠进行压尾机械伤害阈值(Tail flick threshold,TFT)和足底热伤害潜时(Paw withdrawal latency,PWL)两项疼痛行为学测试。注射前1 d,注射后4 h及1~4 d每组随机选取4只大鼠处死,取腰段脊髓,以蛋白印迹法(Western blot)测定NF-κB表达,以酶联免疫吸附法(ELISA)测定PGE2、TNF-α、IL-1β和IL-6的表达。【结果】(1)对照组大鼠行为学(TFT和PWL)及脊髓NF-κBp65、PGE2、IL-1β,TNF-α分子水平与基础值相比无统计学差异。(2)实验组大鼠注射后1h TFT及PWL值升高至最高水平(高于基础水平),随后下降,在注射后第1天降低至最低水平(低于基础水平),随后上升,在第3天恢复至基础水平;脊髓NF-κB、PGE2、IL-1β,TNF-α在注射后4 h、1及2 d表达升高,IL-6在注射后4 h及1 d表达升高。【结论】急性皮下注射芬太尼可引起大鼠的热及机械痛觉过敏和脊髓促炎症因子的升高,且行为学及分子学的改变在时间上平行,提示芬太尼诱导的大鼠痛觉过敏和脊髓炎性反应相关。
[Abstract]:[objective] to investigate the expression of proinflammatory factor in spinal cord of rats induced by fentanyl. [methods] Sixty-four male Sprague-Dawley rats were divided into two groups, the experimental group (Fentanyl group) received 60 渭 g / kg fentanyl subcutaneously, [methods] 64 male Sprague-Dawley rats were injected with fentanyl 60 渭 g / kg for 4 times. Normal saline group (control group) was subcutaneously injected with normal saline 4 times before injection. The rats in each group were given tail flick threshold of mechanical injury (tail flick threshold) and Paw withdrawal latency1 (Paw withdrawal latency1) on the 1st day before injection and 3 hours and 5 days after injection. Pain behavior test. Four rats in each group were randomly selected and killed 1 day before injection, 4 hours after injection and 1 day after injection. The expression of NF- 魏 B in the lumbar spinal cord was determined by Western blot. The expression of IL-1 尾 and IL-6 in PGE2TNF- 伪 TNF- 伪 was determined by Elisa. [results] the levels of TFT and PWL in the control group and the spinal cord NF- 魏 Bp65-PGE2IL-1 尾 -TNF- 伪 increased to the maximum at 1 h after injection. High (above the base level), then down, It was reduced to its lowest level on the first day after injection (below the base level, then rose, and recovered to the base level on the third day; The expression of TNF- 伪 was increased at 4 h and 2 d after injection. [conclusion] Acute subcutaneous injection of fentanyl can induce hyperthermia and mechanical hyperalgesia and increase the level of proinflammatory cytokines in spinal cord. The behavioral and molecular changes were parallel in time, suggesting that fentanyl induced hyperalgesia and spinal cord inflammation in rats.
【作者单位】：广东省第二人民医院麻醉科;中山大学附属第一医院麻醉科;天津市儿童医院麻醉科;广州市番禺区中心医院;
【基金】：国家自然科学基金(81571071) 广东省自然科学基金(2014A030313203)
【分类号】：R614

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