单染与复染在观察SIRT1基因敲除小鼠骨关节炎切片中的差异比较

发布时间：2018-03-15 03:26

本文选题：SIRT基因　切入点：基因敲除　出处：《中国比较医学杂志》2017年10期 　论文类型：期刊论文

【摘要】：目的通过SIRT1基因敲除建立相关小鼠膝关节骨关节炎模型,在此基础上观察各种不同单独染色或复合染色技术在观察软骨组织切片形态学中的差异。方法将小鼠膝关节组织标本分为2组:SIRT1-/-小鼠假手术组(n=6,A组),SIRT1-/-小鼠骨关节炎模型组(n=6,B组)。通过前交叉韧带横断加内侧半月板切除术建立膝关节骨关节炎模型,行HE染色、番红O-固绿染色、番红O-阿利新蓝染色、番红O染色、固绿染色、阿利新蓝染色,6种单独染色或复合染色方式观察膝关节软骨组织形态学变化。结果番红O-固绿染色、番红O-阿利新蓝染色在观察软骨细胞的细胞形态、软骨分层结构的情况、II型胶原纤维的显示、潮线及软骨下骨的改变中,效果更好;而番红O染色、阿利新蓝染色在观察软骨组织缺失方面有一定的优势。结论在观察SIRT1基因敲除小鼠膝关节骨关节炎软骨组织切片中,与单独染色相比,复合染色在获得软骨结构的各种信息方面优势更加明显。
[Abstract]:Objective to establish a mouse model of knee osteoarthritis by SIRT1 knockout. On the basis of this, we observed the differences in the morphology of cartilage sections by different staining techniques or composite staining techniques. Methods the knee joint specimens of mice were divided into two groups: 1: SIRT1-r-a-group in sham-operation group of mice and SIRT1-r-r-SIRT1-r-1 / -mouse bone joint in group A of sham-operation. The model of osteoarthritis of knee joint was established by anterior cruciate ligament transection and medial meniscectomy. He staining, Phanerin O- fixed green staining, Phanerin O- Alixin blue staining, Phanerin O staining, fixed green staining, The morphologic changes of articular cartilage were observed by alisin blue staining or compound staining. Results the morphology of chondrocytes was observed by the staining of Mongren O- solid green and the staining of Phanerin O- Alisin blue, which were used to observe the morphology of chondrocytes, and to observe the morphology of chondrocytes. In the case of the stratified structure of cartilage, the pattern of type II collagen fibers was better in the changes of tidal line and subchondral bone. Alisin blue staining has a certain advantage in detecting cartilage tissue deletion. Conclusion in the tissue section of SIRT1 gene knockout mouse knee osteoarthritis cartilage tissue, compared with the single staining. The advantages of compound staining in obtaining various information about cartilage structure are more obvious.
【作者单位】：滨州市滨城区市立医院;空军总医院;北京大学深圳医院;
【基金】：广东省自然科学基金-博士启动项目(2016A030310070) 深圳市科创委资助项目(JCYJ20160428173412866)
【分类号】：R-332;R684.3

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