miR-365抑制骨肉瘤细胞增殖并促进其凋亡

发布时间：2018-03-18 01:36

本文选题：miR-　切入点：骨肉瘤　出处：《细胞与分子免疫学杂志》2016年01期 　论文类型：期刊论文

【摘要】：目的检测microRNA-365(miR-365)对SOSP-9607骨肉瘤细胞增殖和凋亡的影响。方法利用人工合成的miR-365模拟物以及miR-365抑制物,瞬时转染SOSP-9607骨肉瘤细胞。实时定量PCR(qRT-PCR)检测细胞中miR-365的水平;流式细胞术检测SOSP-9607骨肉瘤细胞的细胞周期与凋亡;MTT法检测细胞增殖能力的变化;qRT-PCR检测细胞中KRAS的水平。结果 miR-365模拟物上调骨肉瘤细胞miR-365表达水平,抑制细胞增殖,细胞G1期增加、S/G2期减少,同时凋亡增加,KRAS基因的表达下降;miR-365抑制物下调SOSP-9607骨肉瘤细胞miR-365的表达水平,促进骨肉瘤细胞增殖,同时抑制凋亡,上调KRAS基因的表达。结论 miR-365抑制SOSP9607骨肉瘤细胞增殖并促进其凋亡,可能通过调节KRAS来发挥抑癌作用。
[Abstract]:Objective to investigate the effect of microRNA-365 miR-365) on proliferation and apoptosis of SOSP-9607 osteosarcoma cells. Methods SOSP-9607 osteosarcoma cells were transiently transfected with synthetic miR-365 mimics and miR-365 inhibitors. Cell cycle of SOSP-9607 osteosarcoma cells and apoptosis of osteosarcoma cells were detected by flow cytometry. The level of KRAS in osteosarcoma cells was detected by qRT-PCR. Results miR-365 mimics upregulated the expression of miR-365 and inhibited the proliferation of osteosarcoma cells. The G 1 phase increased and the S / G 2 phase decreased, while the apoptosis increased and the expression of KRAS gene decreased. MiR-365 inhibitor down-regulated the expression of miR-365 in SOSP-9607 osteosarcoma cells, promoted the proliferation of osteosarcoma cells, and inhibited apoptosis at the same time. Conclusion miR-365 can inhibit the proliferation and promote the apoptosis of SOSP9607 osteosarcoma cells, which may play an inhibitory role by regulating KRAS.
【作者单位】：第四军医大学唐都医院骨科;第四军医大学唐都医院麻醉科;第四军医大学基础部免疫学教研室;第四军医大学唐都医院神经外科;海军总医院消化内科;
【基金】：国家自然科学基金(30901784;81272072)
【分类号】：R738

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