肌肉衰减综合征小鼠模型的建立及相关分子和功能的评价

发布时间：2018-03-18 11:30

本文选题：地塞米松　切入点：肌肉衰减综合征　出处：《锦州医科大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的通过研究地塞米松诱导C57BL/6小鼠产生肌肉质量和肌肉功能的变化,并分析小鼠骨骼肌中相关分子表达水平的变化,探究得到理想的肌肉衰减综合征小鼠的建模方法。方法将35只6~7月龄C57BL/6雄性小鼠分为3组:C组(对照组:0.9%生理盐水)、L组(低剂量组:5 mg/kg地塞米松磷酸钠注射液),H组(高剂量组:10 mg/kg地塞米松磷酸钠注射液),连续皮下注射6周。前2周每3 d以及后4周每7 d测量一次小鼠摄食量、体重和体成分。最后一次给药24 h后,利用水迷宫测量小鼠的游泳速度和轮式跑台测量小鼠的掉落次数,综合评价小鼠的肌肉功能。肌肉功能测试结束后取小鼠腓肠肌,利用荧光定量PCR的方法检测三组小鼠骨骼肌中Atrogin-1、Mu RF1、Myo D和myogenin m RNA的表达水平的差异。结果(1)从造模第1天开始,L组和H组平均每日摄食量较对照组显著增加(P0.005);(2)L组造模第28天和H组造模第21天出现体重较C组显著增加(P0.005)。(3)体成分方面,L组和H组造模第28天出现肌肉质量较C组显著降低(P0.005);L组和H组造模第6天出现脂肪含量较C组显著增加(P0.005)。(4)肌肉功能方面,L组和H组平均游泳速度较C组明显下降(P0.05),平均轮式跑台掉落次数较C组明显增多(P0.05)。(5)L组和H组骨骼肌中Atrogin-1和Mu RF1 m RNA表达较C组均明显升高(P0.05);L组骨骼肌Myo D和Myogenin m RNA表达较C组没有差异,H组骨骼肌中Myo D和Myogenin m RNA表达较C组明显升高(P0.05)。结论通过皮下注射地塞米松磷酸钠,能够建立理想的肌肉衰减综合征小鼠模型。
[Abstract]:Objective to study the changes of muscle mass and muscle function induced by dexamethasone in C57BL / 6 mice and to analyze the expression level of related molecules in skeletal muscle of mice. To explore an ideal method for modeling muscle attenuation syndrome mice, 35 C57BL / 6 male mice aged 6 to 7 months were divided into three groups: group C (control group: 0.9% saline) (low dose group: 5 mg/kg dexamethasone sodium phosphate injection). Group A (high dose group: 10 mg/kg dexamethasone sodium phosphate injection) was injected subcutaneously for 6 weeks, and the food intake of mice was measured every 3 days in the first 2 weeks and every 7 days in the last 4 weeks. Body weight and body composition. After the last administration for 24 hours, the swimming speed of mice was measured by water maze and the falling times of mice were measured by wheeled running platform, and the muscle function of mice was evaluated synthetically. The gastrocnemius muscle of mice was taken after the muscle function test. Fluorescence quantitative PCR was used to detect the expression of Atrogin-1Mu RF1D and myogenin m RNA in skeletal muscle of three groups of mice. Results from the first day of modeling, the average daily intake of Atrogin-1Mu RF1D and myogenin m RNA in group L and group H was significantly higher than that in group B (P 0.005) and group H was significantly higher than that in control group (P 0.005). Body mass in group L and group H on day 28 and group H were significantly lower than those in group C on day 28 and group H were significantly lower than those in group C and group H on day 6. The average swimming speed of group L and group H was significantly lower than that of group C (P 0.05), and the fall times of average wheel running platform were significantly higher than that of group C (P 0.05). The expression of Atrogin-1 and Mu RF1 m RNA in skeletal muscle of group L and H were significantly higher than that of group C. There was no difference in the expression of Myo D and Myogenin m RNA between the two groups. Conclusion the expression of Myo D and Myogenin m RNA in skeletal muscle of H group was significantly higher than that of C group. Conclusion Dexamethasone sodium phosphate can be injected subcutaneously into the skeletal muscle. An ideal mouse model of muscle attenuation syndrome can be established.
【学位授予单位】：锦州医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R685;R-332

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