3D打印骨组织工程支架的制备及其性能评价

发布时间：2018-03-18 13:12

  本文选题：3D打印　切入点：骨组织工程　出处：《暨南大学》2017年硕士论文　论文类型：学位论文

【摘要】：据报道,中国每年因为自然灾害、交通事故、运动损伤等意外造成骨缺损的人数高达300到400万人次,骨组织缺损给患者的日常生活带来立巨大的不便。骨移植是解决骨缺损问题最有效的方法之一,众多的患者需要进行骨修复和骨移植治疗。导致国内外骨修复材料市场的需求日益增加。本研究工作以生物相容性良好的聚己内酯(PCL)和季擕盐(ATPB)、牡蛎壳粉(OSP)为原料,利用3D打印技术分别制备出具有优良成骨效果的聚己内酯/牡蛎壳粉骨组织工程支架和具有抗菌效果聚己内酯/季擕盐/牡蛎壳粉抗菌骨组织工程支架,并采用SEM、FTIR、DSC、力学试验、CCK-8检测以及ALP检测等多种测试表征方法对支架的物理化学性能和生物相容性进行评价,得出的结论如下:(1)通过对双螺杆挤出机以及造粒机的参数进行多次试验后,采用熔融共混挤出方法将粉末状的牡蛎壳粉与聚己内酯颗粒充分混合并挤出造粒,再经由3D打印线材专用挤出机制得聚己内酯/牡蛎壳粉和聚己内酯/季擕盐/牡蛎壳粉抗菌3D打印线材,所制得的3D打印线材粗细均匀,直径为1.75±0.2mm,最佳打印温度为150℃,打印流畅,挤料均匀。(2)利用3D打印技术制得的聚己内酯/牡蛎壳粉骨组织工程支架具有三维连通的多孔结构,SEM、FTIR、DSC、CCK-8检测以及ALP检测等结果表明,牡蛎壳粉在支架中分布均匀,使支架的力学性能、蛋白吸附能力、体外矿化能力均得到改善。支架没有细胞毒性,能有效促进MG63细胞体外碱性磷酸酶(ALP)的表达。动物试验表明支架无毒、无害。(3)利用3D打印技术制得的聚己内酯/季擕盐/牡蛎壳粉骨组织工程支架具有三维连通的多孔结构,CCK-8检测、ALP检测以及抗菌测试等结果表明用牡蛎壳粉负载季擕盐既保持了季擕盐高效的抗菌性能,又降低了它的细胞毒性,在不影响牡蛎壳粉生物活性的前提下提高抗菌剂的生物相容性。动物试验表明支架对动物体无毒、无害。(4)所制得的聚己内酯/牡蛎壳粉骨组织工程支架和聚己内酯/季擕盐/牡蛎壳粉抗菌骨组织工程支架具有良好的生物相容性和生物安全性,有望成为一种具有抗菌性能的骨修复材料。
[Abstract]:According to reports, the number of bone defects caused by natural disasters, traffic accidents, sports injuries and other accidents in China is as high as 300 to 4 million per year. Bone graft is one of the most effective methods to solve the problem of bone defect. A large number of patients need bone repair and bone graft therapy, resulting in a growing demand for bone repair materials at home and abroad. In this study, the biocompatible polycaprolactone (PCLL) and the seasonal salt carrying ATPBN, OSPs, were used as raw materials. The scaffolds for bone tissue engineering of polycaprolactone / oyster shell powder and polycaprolactone / quaternary salt / oyster shell powder scaffolds were prepared by 3D printing technology respectively. The physical and chemical properties and biocompatibility of the scaffolds were evaluated by means of SEM-FTIR DSC, mechanical test, CCK-8 test and ALP test. The conclusions are as follows: (1) after many experiments on the parameters of twin-screw extruder and granulator, the powdered oyster shell powder and polycaprolactone particles were fully mixed and extruded by melt blending extrusion method. Then, polycaprolactone / oyster shell powder and polycaprolactone / quarterly salt / oyster shell powder anti-bacterial 3D printing wire were prepared by special extruder for 3D printing wire. The diameter of 3D printed wire was 1.75 卤0.2 mm, and the optimum printing temperature was 150 鈩，

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