丙泊酚尾静脉泵注对骨髓间充质干细胞移植修复大鼠脊髓损伤的影响

发布时间：2018-03-18 16:38

本文选题：骨髓间充质干细胞　切入点：丙泊酚　出处：《中国老年学杂志》2017年07期 　论文类型：期刊论文

【摘要】：目的检测丙泊酚尾静脉泵注对骨髓间充质干细胞(BMSC)移植修复大鼠脊髓损伤的影响。方法建立80只成年的Wistar大鼠脊髓损伤模型,用随机数字表法分为4组。1BMSC移植组经尾静脉泵注等体积BMSC细胞液。2对照组尾静脉注入培养液组。3丙泊酚组:丙泊酚注射液(2 ml·kg~(-1)·h~(-1))尾静脉滴注4 h;4联合组尾静脉注射BMSC细胞后,经尾静脉泵注丙泊酚注射液(2 ml·kg~(-1)·h~(-1))持续4 h。依次于术前、术后1、3 d与1~4 w通过斜板试验、改良Tarlov评分进行运动功能评定、运动功能量表(BBB)评分。术后4 w取材进行病理切片HE染色及荧光显微镜观测CM-Dil标记的分布及BMSC存活情况。使用荧光金逆行追踪法检测脊髓损伤区神经纤维的再生,并且使用透射电镜法观测神经轴突的生长。结果造模后,大鼠下肢运动功能评价联合组优于BMSC移植组及丙泊酚组,BMSC移植组和丙泊酚组优于对照组,差异均有统计学意义(P0.05)。HE染色,对照组可见脊髓组织缺失及脊髓空洞形成,无神经轴索通过。BMSC移植组和丙泊酚组损伤区可见少量神经轴索样结构,脊髓空洞较小,联合组可见较多神经轴索样结构,未见脊髓空洞。术后4 w,荧光金阳性神经纤维数和CM-Dil阳性细胞:对照组最少,BMSC移植组和丙泊酚组次之,联合组最多,组间差异均有统计学意义(P0.05)。透射电镜观察,BMSC移植组和丙泊酚组横断面正中可见新生的无髓及有髓神经纤维,联合组无髓及有髓神经纤维数多于其他组,对照组电镜下有髓及无髓神经纤维数最少。结论丙泊酚尾静脉泵注可以提高向神经功能细胞分化及移植的BMSC在脊髓损伤区的存活,促进BMSC移植修复大鼠脊髓损伤的效果。
[Abstract]:Objective to investigate the effect of intravenous infusion of propofol on spinal cord injury (sci) repair in rats with bone marrow mesenchymal stem cell (MSCs) transplantation. Methods 80 adult Wistar rats with spinal cord injury were established. The rats were randomly divided into 4 groups: 1 BMSC transplantation group was injected with BMSC cell fluid of equal volume via caudal vein. 2. 3 propofol group: propofol injection 2 ml 路kg ~ (-1) 路hm-1). BMSC cells were injected into caudal vein after 4 h ~ (-1) infusion in combination with caudal vein injection in the control group (2 ml 路kg 路kg ~ (-1)) 路h ~ (-1) BMSC cells were injected via caudal vein. Propofol injection (2 ml 路kg ~ (-1) 路h ~ (-1)) was injected via caudal vein for 4 hours. Motor function was evaluated by modified Tarlov score before operation, 1 ~ 3 days after operation and 1 ~ 4 weeks after operation. The distribution of CM-Dil labeling and the survival of BMSC were observed by fluorescence microscope and HE staining at 4 weeks after operation. The regeneration of nerve fibers in the injured area of spinal cord was detected by fluorescence gold retrograde tracing. The nerve axon growth was observed by transmission electron microscopy. Results the motor function of lower extremity in the combined group was better than that in the BMSC group and propofol group, and propofol group was superior to the control group, and the difference was statistically significant. In the control group, the absence of spinal cord and the formation of syringomyelia were observed. A few axonal structures were observed in the injured area of the group without axonal axon transplantation and propofol group, the size of the syringomyelia was smaller, and more axonal structures were observed in the combined group. No syringomyelia was found. 4 weeks after operation, the number of fluorescent gold positive nerve fibers and CM-Dil positive cells were the least in the control group and the second in the propofol group and the lowest in the control group, and the most in the combined group. There were significant differences between the two groups (P 0.05). The number of unmyelinated and myelinated nerve fibers in the combined group was higher than that in the other groups, and the number of unmyelinated and myelinated nerve fibers in the BMSC transplantation group and propofol group was more than that in the other groups, and the number of unmyelinated and myelinated nerve fibers was higher in the combined group. Conclusion intravenous infusion of propofol via tail vein can improve the survival of BMSC in the injured area of spinal cord and promote the effect of BMSC transplantation in repairing spinal cord injury in rats.
【作者单位】：河北医科大学附属邢台市人民医院麻醉科;河北医科大学附属邢台市人民医院创伤骨科;河北医科大学附属邢台市人民医院手术室;
【分类号】：R614

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