微等离子体射频技术对NB-UVB诱导豚鼠皮肤色素沉着的影响

发布时间：2018-03-20 00:12

  本文选题：紫外线　切入点：豚鼠　出处：《河北医科大学》2016年硕士论文　论文类型：学位论文

【摘要】：目的:微等离子体射频技术(micro-plasma radio-frequency technology)作为一种新技术,近些年来在改善皮肤细纹、粗糙、松弛、瘢痕及色素沉着等治疗中显示出良好的应用前景。临床上,应用微等离子体射频技术治疗瘢痕,从疗效、安全性、恢复时间的总体评估上,有着显著的优越性。在微等离子体射频技术的临床应用中,被证实能显著改善烧伤后瘢痕及皮肤色素沉着。本文通过研究应用微等离子体射频技术治疗窄波中波紫外线(narrow band ultraviolet B,NB-UVB)诱导下豚鼠皮肤色素沉着动物模型,观测与豚鼠皮肤色素沉着相关的酪氨酸酶活性的黑色素细胞数量、黑素颗粒、黑色素细胞功能活性的表达指标,为指导临床治疗瘢痕及色素沉着性疾病提供理论依据。1建立动物模型:选择10只健康豚鼠(普通级),雌性5只,雄性5只,体重0.25~0.35kg,7~10周龄,对豚鼠棕黄色毛发皮肤区域脱毛后行NB-UVB照射,照射剂量:900 m J/cm~2,1次/周,共3次。统计NB-UVB照射前后豚鼠皮肤酪氨酸酶活性的黑色素细胞数目、黑素颗粒面积占表皮面积百分比、c-kit蛋白阳性细胞数目。模型皮肤较正常皮肤各项指标均明显增加,差异有统计学意义(P0.05),可判断为造模成功。2造模成功区域随机分为4组,以不同能量参数的微等离子体射频技术进行治疗:A组:对照组。B、C、D组:治疗组,治疗参数为作用时间0.6s,输出功率依次为10Watt、20Watt、30Watt的微等离子体射频技术照射治疗。分别在治疗前和完成治疗后2、4、6周,取材进行黑色素细胞染色(表皮分离-多巴染色法)、黑素颗粒染色(Masson—Fontana法)、以及免疫组织化学染色(c-kit蛋白),观察酪氨酸酶活性的黑色素细胞数目、黑素颗粒面积占表皮面积百分比、黑色素细胞活性的改变。3应用SPSS13.0统计学软件对酪氨酸酶活性的黑色素细胞数目、黑素颗粒所占表皮面积百分比、c-kit蛋白阳性细胞数目进行统计学分析。NB-UVB照射前后数据采用配对t检验进行比较;微等离子体射频技术治疗结束后2、4、6周各处理组间数据比较,符合正态且方差齐的数据应用区组方差分析,正态且方差不齐或非正态的数据采用区组秩和检验。对各时间点酪氨酸酶活性的黑色素细胞数目、黑素颗粒所占表皮面积百分比进行重复测量的方差分析。以(P0.05)作为差异显著性的判断标准。分析探讨微等离子体射频技术对NB-UVB诱导豚鼠皮肤色素沉着的影响。结果:微等离子体射频技术治疗后色素沉着观测指标的改变1酪氨酸酶活性的黑色素细胞数目结果统计分析:治疗结束后2、4周:B、C、D组较A组低,差异有统计学意义(P0.05);BCD,差异有统计学意义(P0.05)。治疗结束后6周:B、C、D组较A组低,差异有统计学意义(P0.05);B与C组相比差异无统计学意义(P0.05),D组较B、C组低,差异有统计学意义(P0.05)。各时间点重复测量比较,差异有统计学意义(P0.05)。时间因素、分组因素主效应均存在,二者交互作用也存在。2黑素颗粒所占表皮面积百分比变化结果统计分析:治疗结束后2周,B、C、D组较A组低,差异有统计学意义(P0.05);B与C无差异;D组较B、C组低,差异有统计学意义(P0.05)。治疗结束后4周,B、C、D组较A组低,差异有统计学意义(P0.05);C、D组较B组低,差异有统计学意义(P0.05);C与D组相比差异无统计学意义(P0.05)。治疗结束后6周,仅D组低于A组,差异有统计学意义(P0.05);D组较B组低,差异有统计学意义(P0.05);其余组间相比,差异无统计学意义(P0.05)。各时间点重复测量比较,差异有统计学意义(P0.05)。时间因素、分组因素主效应均存在,二者交互作用也存在。3 c-kit蛋白阳性细胞数目结果统计分析:治疗结束后2周,B、C、D组较A组低,差异有统计学意义(P0.05)。治疗结束后4、6周,未发现c-kit蛋白阳性细胞存在。结论:微等离子体射频技术可加速豚鼠皮肤色素沉着的消退,对改善NB-UVB照射后豚鼠皮肤色素沉着的动物模型有效。
[Abstract]:Purpose: micro plasma radio frequency technology (micro-plasma radio-frequency technology) is a new technology in recent years, improve the skin wrinkles, rough, loose, and shows a good prospect in the treatment of scar and pigmentation. Clinically, application of micro plasma radio frequency technology for the treatment of scar, from the curative effect, safety evaluation, overall recovery time, there are significant advantages. In the clinical application of micro plasma radio frequency technology, has been shown to significantly improve the burn scar and skin pigmentation. Through the study on the application of micro plasma radiofrequency for narrow-band UVB (narrow band ultraviolet B, NB-UVB) induced skin pigmentation of guinea pig animal model tyrosinase activity, observation and skin pigmentation related to the number of melanocytes, melanin granules, expression index of melanoma cell function activity .1, provide a theoretical basis for the establishment of animal model for clinical treatment of scar and pigmentation disorders: choose 10 healthy guinea pigs (general), 5 female, 5 male, weight 0.25~0.35kg, 7~10 weeks old, brown hair of guinea pig skin area hair removal NB-UVB after irradiation, irradiation dose: 900 m / J/cm~2,1 week, a total of 3 times. The number of melanocytes of guinea pig skin tyrosinase activity statistics before and after NB-UVB irradiation, epidermal melanin granules area occupies the area percentage, number of c-kit protein positive cells compared to normal skin. Skin model indicators were significantly increased, the difference was statistically significant (P0.05), can be judged as successful model of.2 rats area were randomly divided into 4 groups, were treated by micro plasma radio frequency technology with different energy parameters: group A: control group.B, C, D group: the treatment group, the treatment parameters for the duration of 0.6s, output power: 10Watt, 20Watt, 30W Micro plasma irradiation for the treatment of att. Respectively before treatment and after treatment for 2,4,6 weeks, were analysed by melanin cell staining (epidermal separation - DOPA staining), melanin staining (Masson Fontana method), and immunohistochemical staining (c-kit protein), the number of melanoma cells to observe the activity of tyrosinase melanin granules, area surface area percentage, the number of tyrosinase activity of melanocytes melanin cell activity changes of.3 using SPSS13.0 statistical software, melanin granules for epidermal area percentage, c-kit protein positive cells were analyzed before and after.NB-UVB irradiation data were compared using the paired t test to compare 2,4,6 weeks of each treatment group; the data between the end of micro plasma treatment, with data analysis application area normality and homogeneity of variance, normal distribution and homogeneity of variance or non Normal group data using rank sum test. The number of melanoma cells for each time point of tyrosinase activity, melanin granules for epidermal area percentage of repeated measure ANOVA. In (P0.05) as a criterion of difference. Analysis of the micro plasma radio frequency technology induces skin pigmentation in guinea pigs of NB-UVB. Results: micro plasma after the treatment of pigmentation observation index change of melanocyte tyrosinase activity number results in 1 Statistical Analysis: 2,4 weeks after the treatment: B, C, D group was lower than that of A group, the difference was statistically significant (P0.05); BCD, the difference was statistically significant (P0.05). 6 weeks after the treatment: B, C, D group was lower than that of A group, the difference was statistically significant (P0.05); B group and C group had no significant difference (P0.05), D group compared with B, C group, the difference was statistically significant (P0.05). Each time point of repeated measurement comparison ,宸�寮傛湁缁熻�″�︽剰涔�(P0.05).鏃堕棿鍥犵礌,鍒嗙粍鍥犵礌涓绘晥搴斿潎瀛樺湪,浜岃，

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