CXCL1在重度脑外伤患者中的表达和意义

发布时间：2018-03-20 09:45

本文选题：脑外伤　切入点：CXCL　出处：《中国康复理论与实践》2017年08期 　论文类型：期刊论文

【摘要】：目的研究趋化因子CXCL1在重度脑外伤(TBI)患者脑组织中的细胞定位及在脑组织和血液中的表达,探讨其与损伤严重程度的关系。方法 2013年9月至2015年10月,选取重度TBI住院、且行开颅手术患者78例为实验组,所有患者均收集血液,其中19例收集到脑组织。实验组根据入院时格拉斯哥昏迷量表(GCS)评分分为重型TBI组(6~8分,n=35)和特重型TBI组(3~5分,n=43)。10例病例对照组脑组织标本来自摘除的脑血管瘤或良性肿瘤边缘少量正常脑组织。10例健康对照组血液来自同期在本院健康体检正常者。采用免疫荧光双标法检测重度TBI患者CXCL1在脑组织中的细胞定位;ELISA法检测损伤后脑组织和不同时间点血液中CXCL1蛋白的表达;Spearman相关分析对不同时间点外周血中CXCL1蛋白含量与术后30 d格拉斯哥结局量表(GOS)评分进行相关性分析。结果正常脑组织内CXCL1主要表达在星形胶质细胞;重度TBI后,CXCL1主要表达在神经元和星形胶质细胞。特重型TBI组CXCL1蛋白在脑组织中的含量高于重型TBI组(t=-12.58,P0.05)。重型TBI组CXCL1蛋白在血液中含量于术前达高峰,随后逐渐下降,术后14 d仍高于健康对照组(P0.05),术后30 d降至健康者水平(P0.05)。特重型TBI组CXCL1蛋白在血液中含量术前和术后1 d最高,其后逐渐下降,术后30 d仍高于健康者(P0.05)。术前至术后30 d,特重型TBI组CXCL1蛋白含量均高于重型TBI组(P0.05)。特重型TBI组术前血液中CXCL1蛋白含量与GOS评分呈负相关(r=-0.351,P0.05)。结论重度TBI后CXCL1表达明显增高,且主要表达在神经元和星形胶质细胞,同时CXCL1含量在一定程度上可以反映脑损伤的严重程度和预后。
[Abstract]:Objective to study the chemokine CXCL1 in patients with severe brain injury (TBI) in brain tissue of patients with cellular localization and expression in brain tissue and blood, and explore their relationship with the severity of brain injury. Methods from September 2013 to October 2015, selection of severe TBI in hospital, and underwent craniotomy in 78 patients as the experimental group, all patients were collected the blood, among which 19 cases were collected from brain tissue in experimental group. According to the Glasgow Coma Scale (GCS) scores were divided into severe TBI group (6~8, n=35) and severe TBI group (3~5, n=43).10 cases control group brain tissue samples from the removed edge of cerebral aneurysm or benign tumor a small amount of normal brain tissue.10 controls the blood from the same period in the health examination in our hospital. The normal cellular localization by immunofluorescence assay in patients with severe TBI CXCL1 in brain tissue; brain tissue injury and ELISA assay at different time points The expression of CXCL1 protein in the blood; Spearman correlation analysis on different time points of CXCL1 in peripheral blood and protein content after 30 d Glasgow Outcome Scale (GOS) score were analyzed. Results the normal brain tissue CXCL1 is mainly expressed in astrocytes; severe TBI, CXCL1 mainly expressed in neurons and astrocytes in severe TBI group. CXCL1 protein in brain tissue is higher than in severe TBI group (t=-12.58, P0.05). Severe TBI group CXCL1 protein in blood on the preoperative content reached the peak, then decreased gradually, after 14 d was still higher than that of healthy control group (P0.05), 30 d after the operation to the health level (P0.05). Severe TBI group CXCL1 protein in the blood content of preoperative and postoperative 1 D is the highest, then gradually decreased after 30 d was still higher than that of healthy subjects (P0.05). Preoperative and postoperative 30 d, severe CXCL1 group TBI protein content were higher than severe TBI group (P0.05). Special Severe TBI group preoperative CXCL1 protein content in blood was negatively correlated with the GOS score (r=-0.351, P0.05). Conclusion CXCL1 expression was significantly increased after severe TBI, and mainly expressed in neurons and astrocytes, severity and prognosis and the content of CXCL1 in a certain extent can reflect brain damage.

【作者单位】：南通大学附属医院康复医学科;常州市妇幼保健院;
【基金】：南通大学博士科研启动基金项目(No.2016-2) 南通市科技计划项目(No.MS22016044)
【分类号】：R651.15

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