巨噬细胞移动抑制因子及其拮抗剂ISO-1在病理性瘢痕发病机制中的作用

发布时间：2018-03-21 02:45

本文选题：巨噬细胞移动抑制因子　切入点：病理性瘢痕　出处：《实用医学杂志》2017年03期 　论文类型：期刊论文

【摘要】：目的:探究巨噬细胞移动抑制因子(MIF)参与病理性瘢痕发病的具体机制,以及其拮抗剂ISO-1对病理性瘢痕来源成纤维细胞的影响。方法:收集正常皮肤、正常瘢痕以及病理性瘢痕标本,行HE以及免疫组化染色。利用组织块培养法分离培养人成纤维细胞,予以不同浓度ISO-1干预(0~100μmol/mL)。TUNEL染色检测细胞凋亡,Western blot以及RT-PCR检测成纤维细胞特异性蛋白以及PI3K/Akt/mTOR通路的表达情况。结果:MIF在增生性瘢痕、瘢痕疙瘩中表达强于正常瘢痕和正常皮肤。无干预组凋亡细胞较少,予以不同浓度ISO-1干预后,凋亡率逐渐上升。各组各时间点组间迁徙率比较差异均具有统计学意义(P0.05)。随着干预浓度上升,Ⅰ型胶原蛋白、纤连蛋白以及结缔组织生长因子的蛋白和mRNA表达量均下降。活化PI3K以及活化Akt表达量随着ISO-1浓度增加而下降。结论:MIF在不同类型瘢痕组织中表达存在差异,ISO-1通过PI3K/Akt/mTOR通路抑制成纤维细胞生物学行为。
[Abstract]:Objective: to investigate the mechanism of macrophage migration inhibitory factor (MIF) involved in the pathogenesis of pathological scar and the effect of its antagonist ISO-1 on fibroblasts derived from pathological scar. Methods: normal skin was collected. Normal and pathological scar specimens were stained with HE and immunohistochemistry. Human fibroblasts were isolated and cultured by tissue mass culture. Different concentrations of ISO-1 were used to detect the expression of specific protein and PI3K/Akt/mTOR pathway in fibroblasts by Western blot and RT-PCR. The expression of apoptotic cells in keloid was stronger than that in normal scar and normal skin. The rate of apoptosis increased gradually. There were significant differences in migration rates among the groups at different time points (P 0.05). With the increase of the concentration of intervention, type I collagen protein increased. The expression of fibronectin and connective tissue growth factor (connective tissue growth factor) protein and mRNA decreased. The expression of activated PI3K and activated Akt decreased with the increase of ISO-1 concentration. PI3K/Akt/mTOR pathway inhibits the biological behavior of fibroblasts.
【作者单位】：广东省深圳市龙华新区中心医院烧伤整形外科;
【分类号】：R622

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