HIF-1α介导BMSCs复合PLGA修复大鼠颅骨标准骨缺损的研究

发布时间：2018-03-21 11:49

本文选题：低氧诱导因子-α　切入点：骨髓间充质干细胞　出处：《安徽医科大学学报》2017年09期 　论文类型：期刊论文

【摘要】：目的构建低氧诱导因子(HIF)-1α介导骨髓间充质干细胞(BMSCs)复合聚乳酸-聚乙醇酸共聚物(PLGA)支架材料血管化组织工程骨,观察其在大鼠颅骨标准骨缺损中的修复效果。方法将目的基因HIF-1α转染至BMSCs后,与支架材料PLGA复合,修复SD大鼠颅骨双侧直径5 mm的标准骨缺损(n=18),随机分为3组:实验组植入HIF-1α-BMSCs/PLGA复合体(n=6),对照组植入BMSCs/PLGA复合体(n=6),材料组仅植入PLGA支架材料(n=6)。术后8周处死大鼠取材,分别行大体观察、X线片检查和HE染色观察缺损区骨形成情况。结果大体观察、X线片检查和HE染色均显示实验组缺损区新骨形成量明显大于对照组及材料组,差异有统计学意义(P0.05)。结论体内研究表明HIF-1α能够介导BMSCs促进骨组织形成,PLGA是理想的支架材料,用其构建的血管化工程骨能够有效修复骨缺损。
[Abstract]:Objective to construct hypoxia inducible factor (HIF) -1 induced by bone marrow mesenchymal stem cells (BMSCs) compound poly lactic acid glycolic acid (PLGA) scaffolds for tissue engineering of vascularized bone in the skull, observe the standard bone defect in rats. Methods the repair effect of HIF-1 gene transfection to BMSCs alpha PLGA, composite and scaffold materials, standard bone defect repair SD rat skull bilateral 5 mm diameter (n=18), were randomly divided into 3 groups: the experimental group was implanted with HIF-1 alpha -BMSCs/PLGA complex (n=6), the control group was implanted with BMSCs/PLGA complex (n=6), materials were implanted into PLGA scaffolds (n=6) after 8. Weeks the rats were killed, respectively, for gross observation, X-ray examination and HE staining bone formation. The gross observation, X-ray examination and HE staining showed that the experimental group defect new bone formation was significantly greater than that of the control group and material group, the difference was statistically significant (P0.05). Conclusion in vivo studies indicate that HIF-1 alpha can mediate BMSCs to promote bone formation, PLGA is an ideal scaffold material, and its construction of vascular chemical process bone can effectively repair bone defects.

【作者单位】：安徽医科大学口腔医学院;安徽医科大学附属口腔医院安徽省口腔疾病研究中心实验室;
【基金】：国家自然科学基金(编号:81371114) 安徽省自然科学基金(编号:1408085MKL29) 安徽省科技攻关项目(编号:1604a0802082)
【分类号】：R68

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