A型肉毒素预防人瘢痕成纤维细胞增生机制的初步研究

发布时间：2018-03-22 10:16

本文选题：肉毒杆菌毒素　切入点：A型　出处：《重庆医学》2017年36期 　论文类型：期刊论文

【摘要】：目的探究不同浓度A型肉毒素(BTX-A)对瘢痕成纤维细胞的影响,初步阐释BTX-A治疗瘢痕及预防术后瘢痕增生的相关分子作用机制。方法选取人瘢痕成纤维细胞,以不同浓度的BTX-A(0.01、0.10、1.00U/L及10.00U/L)作用24h后,激光共聚焦显微镜观察细胞黏附及骨架变化,并采用MTT及流式技术检测其增殖、凋亡及周期变化,同时采用实时荧光定量PCR(qRCR)及蛋白免疫印迹(Western blot)方法,探究TGF-β、基质金属蛋白酶(MMP)-1、MMP-2及MMP-9基因及蛋白的表达变化。结果随着BTX-A剂量的升高,其细胞黏附数量及骨架荧光强度逐渐减弱;细胞增殖能力减弱且主要阻断在细胞G0/G1期;此外其凋亡也随着BTX-A剂量增大而逐渐增强。qPCR及Western blot结果显示,随着BTX-A剂量增大,MMP-1及MMP-2基因及蛋白均呈现高表达,而TGF-β及MMP-9呈现出低表达。结论 BTX-A通过阻断瘢痕细胞G0/G1期抑制其增殖,同时提高MMP-1及MMP-2的表达来减轻瘢痕形成,对瘢痕的治疗起着积极的作用。
[Abstract]:Objective to explore the different concentrations of type A botulinum toxin (BTX-A) fibroblasts of scar, a preliminary explanation of BTX-A treatment and prevention of postoperative scar related molecular mechanism. Methods hypertrophic scar fibroblasts were, with different concentrations of BTX-A (0.01,0.10,1.00U/L and 10.00U/L) after 24h, to observe the changes of cell adhesion and cytoskeleton laser confocal microscopy, and using MTT and flow cytometry to detect the proliferation, apoptosis and cycle of change, at the same time by using real-time quantitative PCR (qRCR) and Western blot (Western blot) method, explore TGF- beta, matrix metalloproteinase (MMP) -1, MMP-2 and MMP-9 gene expression and protein. Results with the the dose of BTX-A increased, the number of cell adhesion and cytoskeletal fluorescence intensity decreased; cell proliferation decreased and mainly blocked in G0/G1 phase cells; in addition to its apoptosis with increasing doses of BTX-A by Western gradually enhanced.QPCR and blot results showed that with increasing doses of BTX-A, high expression of MMP-1 and MMP-2 gene and protein were present, while TGF- and MMP-9 showed a low beta expression by blocking the scar. Conclusion BTX-A inhibit the proliferation of cells in G0/G1 phase, and increase the expression of MMP-1 and MMP-2 to reduce the scar formation, plays a positive role to treat scar.

【作者单位】：重庆市中医院皮肤美容科;
【基金】：重庆市卫计委医学科研项目(20142071)
【分类号】：R622

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