皮质酮促进大鼠成骨细胞内脂质积聚的量效与时效作用的观察

发布时间：2018-03-25 23:29

本文选题：成骨细胞　切入点：皮质酮　出处：《中国骨质疏松杂志》2017年09期

【摘要】：目的探讨皮质酮作用下离体SD大鼠成骨细胞SREBP(固醇调节元件结合蛋白,sterol modulates the element binding protein)1、SREBP2、HMGCR(3-羟基-3-甲基戊二酸单酰辅酶A还原酶,3-hydroxy-3-methyl malonate coenzyme A reductase)、FAS(脂肪酸合成酶,fatty acid synthase)的表达改变对细胞内脂质积聚的影响。方法采用多次胶原酶组织消化法获得新生SD大鼠颅骨中的成骨细胞作为研究对象,观察皮质酮作用后成骨细胞内脂质积聚的特征,以及SREBP1、SREBP2、HMGCR、FAS基因及蛋白表达。结果成骨细胞内Nile Red细胞内脂质染色可见成骨细胞内的脂质染色随皮质酮浓度的增加的逐渐增多,相同浓度的皮质酮在作用的24 h、48 h、72 h后未见明显差异;细胞内SREBP1、SREBP2、HMGCR、FAS基因表达在不同浓度皮质酮作用24 h后均明显增高,48 h、72 h后呈现下降趋势;细胞内SREBP1、SREBP2、HMGCR、FAS蛋白在1μmol/L皮质酮作用24 h后表达升高,48 h后与对照组未见明显差异,72 h后SREBP1、FAS表达降低,SREBP2表达升高,HMGCR未见明显差异。结论超生理剂量的皮质酮能促进成骨细胞内脂质异常沉积;不同浓度皮质酮作用成骨细胞24 h后能促进细胞内SREBP1、SREBP2、HMGCR、FAS基因表达;皮质酮作用成骨细胞24 h后促进成骨细胞内SREBP1、SREBP2、HMGCR、FAS蛋白的表达。
[Abstract]:Objective to investigate the expression of 3-hydroxy-3-methyl malonate coenzyme A reductase A reductase (3-hydroxy-3-methyl malonate coenzyme A reductase) in osteoblasts of SD rats induced by corticosterone in vitro, and to investigate the expression and modification of fatty acid synthase fatty acid synthase (fatty acid synthase). Methods osteoblasts from the skull of newborn SD rats were obtained by multiple collagenase tissue digestion. To observe the characteristics of lipid accumulation in osteoblasts and the expression of HMGCR-FAS gene and protein in osteoblasts treated with corticosterone, lipid staining in Nile Red cells showed that lipid staining in osteoblasts increased with the increase of corticosterone concentration. There was no significant difference between the same concentration of corticosterone at 24 h, 48 h and 72 h, but the expression of SREBP1 + SREBP2HMGCR-FAS gene in the cells increased significantly after 24 h of treatment with different concentrations of corticosterone, and decreased after 72 h of treatment with different concentrations of corticosterone. The expression of SREBP1HMGCR-FAS protein increased after 24 h treatment with 1 渭 mol/L corticosterone. There was no significant difference in expression of SREBP1FAS between the cells and the control group after treatment with 1 渭 mol/L for 24 h. Conclusion there is no significant difference in the expression of SREBP1FAS after 72 h. Conclusion the hyperphysiological dose of corticosterone can promote osteogenesis. Abnormal lipid deposition in cells; Different concentrations of corticosterone could promote the expression of HMGCR-FAS gene in osteoblasts after 24 h treatment with different concentrations of corticosterone, and promote the expression of FAS protein in osteoblasts with SREBP1- SREBP2- HMGCR-FAS protein after 24 h treatment with corticosterone.
【作者单位】：广东医科大学附属医院骨科中心;
【基金】：广东省科技计划项目(2011B031800172) 湛江市非资助科技攻关计划项目(2014B01077)
【分类号】：R68

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