亚麻醉、镇静及麻醉剂量丙泊酚对高脂血症大鼠空间记忆及海马组织的影响

发布时间：2018-03-26 15:32

本文选题：丙泊酚　切入点：高脂血症　出处：《中国现代医学杂志》2017年13期

【摘要】：目的探讨亚麻醉、镇静及麻醉剂量丙泊酚对高脂血症大鼠的空间记忆及海马组织的影响。方法65只健康成年雄性SD大鼠随机分为基础性喂养组(15只)和高脂饲料喂养组(50只),喂养6周后随即抽取10只测定血脂。将复制模型成功的40只大鼠随机分为4组:A组腹腔注射生理盐水75 mg/kg;B、C、D组分别腹腔注射亚麻醉(10 mg/kg)、镇静(30 mg/kg)及麻醉剂量(375 mg/kg)丙泊酚(浓度为1%),连续给药5 d,给药后20 min以及第6~7天均进行Morris水迷宫训练,测定空间记忆能力;给药后第8天,断头取脑,获得海马组织,测定其中Tau蛋白磷酸化水平、Caspase-3表达水平和γ氨基丁酸(GABA)水平。结果随着给药和训练天数增加,4组大鼠的逃避潜伏期缩短(P0.05);C组和D组大鼠不同时间点的逃避潜伏期高于A组和B组(P0.05),且D组高于C组(P0.05);第7天空间搜索实验时,C组和D组大鼠穿越平台次数低于A组和B组(P0.05),且D组低于C组(P0.05);C组和D组大鼠海马组织中GABA含量、阳性细胞平均光密度值高于A组和B组(P0.05),且D组高于C组(P0.05)。结论镇静和麻醉剂量的丙泊酚会降低高脂血症大鼠的空间记忆能力,可能与海马组织中GABA的高水平和Tau蛋白过度磷酸化有关。
[Abstract]:Objective to study subanesthesia, Effects of sedation and anesthetic dose propofol on spatial memory and hippocampal tissue in hyperlipidemic rats. Methods Sixty-five healthy adult male SD rats were randomly divided into basic feeding group (n = 15) and hyperlipidemic diet group (n = 50). After 6 weeks, 10 rats were randomly divided into 4 groups: group A: normal saline 75 mg / kg intraperitoneal injection of normal saline 75 mg / kg, group D received intraperitoneal injection of subanaesthesia 10 mg / kg, sedation 30 mg / kg) and anesthetic dosage 375 mg / kg) propofol. Morris water maze training was performed 20 min and 6 ~ 7 days after continuous administration for 5 days. On the 8th day after administration, the brain was taken from the head and the hippocampus was obtained. The level of Tau protein phosphorylation and the level of GABA were measured. Results with the increase of drug administration and training days, the escape latency of rats in group C and group D was shortened at different time points. On the 7th day, the frequency of crossing platform in group C and group D was lower than that in group A and group B, and the content of GABA in hippocampus of group D was lower than that of group C and group D. The average optical density of positive cells was higher than that of group A and group B, and that of group D was higher than that of group C. Conclusion sedative and anesthetic doses of propofol can reduce the spatial memory ability of hyperlipidemic rats. It may be related to the high level of GABA and excessive phosphorylation of Tau protein in hippocampus.
【作者单位】：海南医学院第二附属医院麻醉科;海南医学院第一附属医院解剖教研室;
【分类号】：R614

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