人瘢痕组织脱细胞真皮基质的组织结构和生物力学性能

发布时间：2018-03-27 22:26

本文选题：瘢痕　切入点：脱细胞真皮基质　出处：《青海大学》2015年硕士论文

【摘要】：目的:观察人瘢痕组织脱细胞真皮基质(ADM)的组织结构和生物力学性能。方法:取8例人成熟瘢痕、8例人增生性瘢痕和4例人正常皮肤,分别将其真皮层用鼓式取皮刀制备成约0.5 mm厚皮片,用2.5 g/L胰蛋白酶及体积分数0.5%Triton X-100脱细胞处理制造出ADM;然后通过大体、组织学HE染色、扫描电镜观察其组织结构的差别,并分别进行离体接种人表皮细胞培养,观察其细胞黏附生长情况的不同;最后通过检测每种ADM的应力-应变关系、应力松弛、蠕变和最大拉伸应力,分析其生物力学性能的差异。结果:制备的ADM均呈瓷白色,正常皮肤和成熟瘢痕的ADM质地柔软、有弹性,而增生性瘢痕的ADM质地稍差;光学显微镜和扫描电镜下可见各种ADM均无细胞存在,正常皮肤ADM胶原纤维均匀、排列规则有序,成熟瘢痕ADM胶原纤维粗细不等、排列稍紊乱,增生性瘢痕ADM胶原纤维排列较为致密紊乱;离体接种人表皮细胞2周后三种ADM上均有表皮细胞黏附生长,在正常皮肤和成熟瘢痕ADM组织表面和内部呈聚集生长,在增生性瘢痕ADM组织表面呈黏附生长;增生性瘢痕、成熟瘢痕、正常皮肤三种ADM的应力-应变参数β值(3.024±0.413、2.595±0.443、2.590±0.366)、蠕变斜率(0.018±0.003、0.019±0.009、0.023±0.010)和最大拉伸应力(8.971±2.434、11.011±1.492、15.567±2.931)差异均无统计学意义(均P㧐0.05);成熟瘢痕与正常皮肤ADM的应力-应变伸长比(0.238±0.083与0.291±0.048)、松弛斜率(-0.041±0.009与-0.047±0.008)、松弛量(0.775±0.194与0.968±0.211)、蠕变量(0.033±0.022与0.049±0.020)差异均无统计学意义(均P㧐0.05),而增生性瘢痕ADM的上述指标(0.188±0.036、-0.033±0.006、0.481±0.058、0.020±0.005)均显著低于正常皮肤ADM(均P0.05)。结论:成熟瘢痕ADM的组织结构、生物力学性能与正常皮肤ADM近似,其生物力学性能优于增生性瘢痕ADM。
[Abstract]:Objective: To observe the effects of scar tissue of acellular dermal matrix (ADM) structure and biomechanical properties. Methods: 8 cases of mature scar, 8 cases of hypertrophic scar and 4 cases of normal skin, the dermis with drum dermatome prepared into 0.5 mm thick skin piece, with 2.5 g /L of pancreas the volume fraction of 0.5%Triton protease and X-100 acellular processing manufacturing ADM; then by gross and histological HE staining to observe the structure difference of scanning electron microscope, and were inoculated with in vitro cultured human epidermal cells, observe the cell adhesion and growth situation of different; finally through the detection of each ADM stress-strain relationship. Stress relaxation, creep and the maximum tensile stress, difference analysis of its biomechanical properties. Results: the preparation of ADM was porcelain white, normal skin and scar ADM texture is soft, elastic, and hypertrophic scar ADM texture is poor; optics All visible under the microscope and scanning electron microscope ADM showed no cells, normal skin collagen fibers of ADM uniform, ordered, mature scar collagen fiber thickness ranging from ADM, arranged a little disorder. ADM of hypertrophic scar collagen fibers arranged in more compact disorder; in vitro inoculation of human epidermal cells after 2 weeks of three ADM on the adhesion of epidermis cell growth, showed aggregation growth in normal skin and scar tissue and internal surface of ADM, ADM in hypertrophic scar tissue surface was adhesion growth; hypertrophic scar, scar, value of stress and strain parameters of normal skin of three ADM (3.024 + 0.413,2.595 + 0.443,2.590 + 0.366 (0.018 +), slope creep 0.003,0.019 + 0.009,0.023 + 0.010) and the maximum tensile stress (8.971 + 2.434,11.011 + 1.492,15.567 + 2.931) there were no significant differences (P? 0.05); mature scar and normal skin ADM stress strain (elongation ratio 0.238 + 0.083 and 0.291 + 0.048), relaxation slope (-0.041 + / - 0.009 and -0.047 + 0.008), (0.775 + 0.194 slack and 0.968 + 0.211), creep (0.033 + 0.022 and 0.049 + 0.020) there were no significant differences (P? 0.05), and the index of hypertrophic scar ADM (0.188 + 0.036, -0.033 + 0.006,0.481 + 0.058,0.020 + 0.005) was significantly lower than that in normal skin ADM (P0.05). Conclusion: the tissue structure of mature ADM scar and normal skin, the biomechanical properties of the ADM approximation, the better biomechanical property of hypertrophic scar ADM.

【学位授予单位】：青海大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R622.1

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