音猬因子修饰纳米晶胶原基骨复合骨髓间充质干细胞修复股骨缺损

发布时间：2018-03-29 15:01

本文选题：骨移植　切入点：干细胞　出处：《中国组织工程研究》2017年14期

【摘要】：背景:音猬因子作为一种Hedgehog信号蛋白,具有促使骨组织发育、生长及重构等生理功能。目的:观察音猬因子修饰的纳米晶胶原基骨复合骨髓间质干细胞修复大鼠股骨缺损的效果。方法:将48只SD大鼠随机分为4组,均建立股骨缺损模型,造模成功后14 d,实验组于骨缺损处植入音猬因子修饰的纳米晶胶原基骨-骨髓间充质干细胞复合物,支架组于骨缺损处植入纳米晶胶原基骨,细胞支架组植入纳米晶胶原基骨-骨髓间充质干细胞复合物,空白对照组不植入任何材料。修复后3,6,9,12周,进行骨缺损区X射线检查、骨密度及骨组织病理切片观察。结果与结论:(1)X射线检查:实验组修复后不同时间点的Lane-Sandhu X射线评分、骨密度值均高于其余3组(P0.05);(2)苏木精-伊红染色:修复后12周,支架组有少量骨组织形成,无骨髓形成;细胞支架组有少量骨组织形成,无骨髓形成,可见残留支架;实验组植入物完全吸收,成熟骨组织和髓腔形成,腔内有骨髓形成;(3)扫描电镜观察:修复后12周,支架组有不规则排列的骨纤维和大量骨陷下窝;细胞支架组可见大量成骨细胞,但骨纤维仍然不规则;实验组有大量排列规则的骨小梁,可见哈佛系统;(4)结果表明:音猬因子修饰的纳米晶胶原基骨复合骨髓间质干细胞可促进骨缺损的修复。
[Abstract]:Background: as a Hedgehog signal protein, Hedgehog plays an important role in bone tissue development. Objective: to observe the effect of nanocrystalline collagen modified bone marrow mesenchymal stem cells (BMSCs) modified by hedgehog factor on the repair of femur defect in rats. Methods: 48 SD rats were randomly divided into 4 groups to establish femoral defect models. On the 14th day after successful modeling, the experimental group was implanted with nanocrystalline collagen-bone mesenchymal stem cell complex modified by hedgehog factor in the bone defect, and the scaffold group was implanted with nanocrystalline collagen-derived bone in the bone defect. The scaffold group was implanted with nanocrystalline collagen-bone mesenchymal stem cell complex, while the blank control group was not implanted with any material. Results and conclusion the Lane-Sandhu X-ray score at different time points after repair in the experimental group was higher than that in the other three groups (P0.05 / 2) hematoxylin-eosin staining: 12 weeks after repair, the bone mineral density of the experimental group was higher than that of the other three groups. The scaffold group had a small amount of bone tissue formation, no bone marrow formation, no bone marrow formation, there was no bone marrow formation, and the experimental group had completely absorbed the implants, and the mature bone tissue and medullary cavity were formed. Scanning electron microscope observation: at 12 weeks after repair, there were irregular arrangement of bone fibers and a large number of bone lacunae in the scaffold group, a large number of osteoblasts were observed in the cell scaffold group, but the bone fibers were still irregular in the scaffold group. There were a large number of regular trabeculae in the experimental group. The results showed that the Hedgehog factor modified nanocrystalline collagen-based bone and bone marrow mesenchymal stem cells could promote the repair of bone defect.
【作者单位】：中山大学附属第五医院骨一科;
【分类号】：R318.08;R683

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