不同浓度七氟烷麻醉对大鼠高氧性肺损伤的影响

发布时间：2018-03-30 15:13

本文选题：七氟烷　切入点：高氧性肺损伤　出处：《川北医学院》2017年硕士论文

【摘要】：目的:比较不同浓度七氟烷麻醉对大鼠高氧性肺损伤的影响。方法:1、使用SPSS17.0随机数字发生器将60只大鼠分为空白对照组(C组,10只)和实验组(S组,50只)。S组使用5L/min左右的氧气,浓度维持95%以上,暴露时间48小时,复制高氧性肺损伤大鼠模型;2、使用SPSS17.0随机数字发生器将S组的50只大鼠分为5组,分别吸入0%、1%、1.5%、2%、2.5%的七氟烷1小时,并分别命名为S0、S1.0、S1.5、S2.0、S2.5组;3、在完成高氧处理48小时(T1)、七氟烷吸入1小时(T2)两个时间点,经10%的水合氯醛麻醉下经腹主动脉抽取动脉血、血清及收集左右肺组织;4、动脉血行血气分析PO2、PCO2;使用ELISA法检测血清TNF-α、IL-8和IL-6浓度;取左肺组织进行湿/干(Wet/dry,W/D)比测量;取右肺组织进行HE染色观察病理损伤程度并进行评分。评分参考指标:肺泡腔充血、出血、肺泡腔或血管壁中性粒细胞浸润聚集、肺泡壁增厚和透明膜形成等。结果:1、在高氧处理48小时后(T1),与C组比较,S组的PO2降低、PCO2增加、TNF-α、IL-8和IL-6浓度增加、W/D比值增加,病理损伤评分增加,差异具有显著性(P0.05);S0至S2.5组组间两两比较,上述指标均无显著性差异(P0.05)。2、经七氟烷处理1小时后(T2):(1)与C组比较,S组的PO2降低、PCO2增加、TNF-α、IL-8和IL-6浓度增加、W/D比值增加,病理损伤评分增加,差异具有显著性(P0.05);(2)与七氟烷处理前比较,S1至S2.5组的PO2增加、PCO2降低、TNF-α、IL-8和IL-6浓度降低、W/D比值降低,病理损伤评分降低,差异具有显著性(P0.05),S0组上述指标无显著性改变(P0.05);(3)与S2.0组比较,S1.0与S1.5组的PO2降低、PCO2增加、TNF-α、IL-8和IL-6浓度增加、W/D比值增加,病理损伤评分增加,差异具有显著性(P0.05);(4)与S2.0组比较,S2.5组PO2、PCO2、TNF-α、IL-8和IL-6浓度、湿/干比值及病理学评分无显著性差异(P0.05)。结论:1、使用95%浓度氧气48小时可复制高氧性肺损伤大鼠模型;2、七氟烷可以有效降低大鼠高氧导致的肺损伤程度;3、2%浓度七氟烷对大鼠高氧性肺损伤有较好的保护效应。
[Abstract]:Objective: to compare the effects of sevoflurane anesthesia at different concentrations on hyperoxia-induced lung injury in rats. Methods: #number0# rats were randomly divided into control group (n = 10) and control group (n = 50) and group S (n = 50). The rat model of hyperoxia lung injury was established by using SPSS17.0 random number generator. The 50 rats in group S were divided into 5 groups and inhaled with 1.5% and 2.5% sevoflurane for 1 hour, respectively. They were named S0 S1.0S1.0S1.5S2.0S2.5 groups. After 48 hours of hyperoxia treatment and 1 hour of sevoflurane inhalation, arterial blood was drawn from the abdominal aorta under 10% chloral hydrate anesthesia at two time points, one hour after hyperoxia treatment, and one hour after inhalation of sevoflurane, and the arterial blood was extracted from the abdominal aorta under 10% chloral hydrate anesthesia. Serum and left and right lung tissues were collected and arterial blood was used to analyze PO2PCO2. Serum TNF- 伪 IL-8 and IL-6 were detected by ELISA method. Wet / dry Wet / dry WR / D ratio was measured in left lung tissue. The right lung tissue was stained with HE to observe the degree of pathological injury and to grade it. The reference indexes were as follows: congestion, hemorrhage, neutrophil infiltration and aggregation in alveolar cavity or vascular wall. Results the alveolar wall thickening and hyaline membrane formation were observed after hyperoxia treatment for 48 hours. Compared with group C, PO2 decreased, PCO2 increased, TNF- 伪 -IL-8 and IL-6 concentration increased, and pathological injury score increased. There was significant difference between group S0 and S2.5. There was no significant difference in the above indexes. After 1 hour of sevoflurane treatment, the PO2 of group S was decreased, and the concentration of TNF- 伪 IL-8 and IL-6 increased, the ratio of TNF- 伪 IL-8 and IL-6 increased, the score of pathological injury increased, compared with group C, the ratio of TNF- 伪 IL-8 and TNF- 伪 IL-8 and the ratio of TNF- 伪 IL-8 and IL-6 increased, and the pathological injury score increased after 1 hour of sevoflurane treatment. Compared with sevoflurane treatment, PO2 increased in S1-S2.5 group and decreased in TNF- 伪 -IL-8 and IL-6 concentration. The ratio of TNF- 伪, IL-8 and IL-6 decreased, and the pathological injury score decreased, compared with that of sevoflurane treatment. There was no significant difference in the above indexes between S0 group and S0 group. Compared with S2.0 group, the decrease of PO2 in S1.0 and S1.5 groups increased the concentration of TNF- 伪 IL-8 and IL-6, and the ratio of TNF- 伪 IL-8 and IL-6 increased, and the pathological injury score increased. The concentrations of IL-8 and IL-6 in group S2.5 were significantly higher than those in group S2.0, and the concentration of IL-8 and TNF- 伪 in group S2.5 were significantly higher than that in group S2.0. There was no significant difference in wet / dry ratio and pathological score (P 0.05). Conclusion: 95% oxygen for 48 hours can induce hyperoxia-induced lung injury in rats. Sevoflurane can effectively reduce the degree of lung injury induced by hyperoxia in rats. Halothane has a better protective effect on hyperoxic lung injury in rats.
【学位授予单位】：川北医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R614

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