白藜芦醇对新生SD大鼠七氟烷麻醉后认知功能改变的影响

发布时间：2018-03-31 02:20

本文选题：白藜芦醇　切入点：七氟烷　出处：《重庆医科大学》2017年硕士论文

【摘要】：目的:探讨白藜芦醇(Resveratrol,RES)预处理对新生SD大鼠七氟烷麻醉后认知功能改变的影响。方法:将新生SD大鼠随机分为对照(Con)组,白藜芦醇(RES)组,七氟烷(Sevo)组,七氟烷+白藜芦醇(Sevo+RES)组。采用自制的麻醉箱将新生6天(P6)的SD大鼠暴露于2.5%的七氟烷环境中,持续3天,每天2 h,建立七氟烷暴露模型,每次七氟烷暴露前30min给予腹腔注射白藜芦醇(30mg/kg)。对照组与白藜芦醇组则暴露于除七氟烷外其余条件相同的环境中。于第3天暴露结束后(P8)立即处死,取脑组织,HE染色观察脑组织病理形态学变化;实时荧光聚合酶链反应(Real-time polymerase chain reaction,RT-PCR)检测海马组织中Sirt1、PGC-1α及FOXO3αm RNA的表达;免疫印迹法(Western blotting,WB)检测海马中沉默信息调节因子2相关酶I(Silent information adjustment factor 2 related enzyme I,Sirt1)及半胱氨酸天冬氨酸蛋白酶(Cysteiny1 aspartate-specific proteinase,Caspase3)的蛋白表达;超氧化物歧化酶(Superoxide Dismutase,SOD)和丙二醛(Malonaldehyde,MDA)试剂盒化学法检测海马组织匀浆中SOD、MDA活性变化;第30天(P30)进行Morris水迷宫实验评价各组大鼠学习记忆能力。结果:与Con组比较,Sevo组神经组织明显疏松,水肿,部分胞浆溶解,核固缩、碎裂,给予RES预处理七氟烷暴露(Sevo+RES组)后,也可见细胞水肿,核固缩等病理形态,但程度较Sevo组明显减轻;与Con组比较,Sevo组Sirt1、PGC-1α、FOXO3αm RNA的表达均降低(P0.05),SOD活性降低(P0.05),MDA水平升高(P0.05);而与Sevo组相比,Sevo+RES组Sirt1、PGC-1α及FOXO3αm RNA表达明显增强(P0.05),SOD活性增高(P0.05),MDA水平降低(P0.05)。Western blot结果显示Sevo组与Sevo+RES组Sirt1表达均低于Con组(P0.05),Sevo组Caspase3与Con组相比则明显增加(P0.05);但是与Sevo组相比,Sevo+RES组Sirt1表达升高(P0.05),Caspase3表达降低(P0.05)。Morris水迷宫实验提示各组间在逃避潜伏期,穿越平台次数以及目标象限停留时间上均无明显差异(P0.05)。结论:白藜芦醇可以降低氧化应激水平,减轻七氟烷暴露所致新生SD大鼠脑损伤,但是,不论是否给予白藜芦醇预处理,七氟烷对于SD大鼠的远期学习记忆能力无明显损害。
[Abstract]:Objective: to investigate the effect of resveratrol resveratrolone on cognitive function of neonatal SD rats after sevoflurane anesthesia. Methods: neonatal SD rats were randomly divided into control group, resveratrol group and sevoflurane Sevogroup. Sevoflurane resveratrol Sevo res) group. SD rats of 6 days old were exposed to 2.5% sevoflurane for 3 days, 2 hours a day, to establish sevoflurane exposure model. Before each sevoflurane exposure, 30min was given intraperitoneal injection of resveratrol 30 mg / kg. The control group and resveratrol group were exposed to the same conditions except sevoflurane. The histopathologic changes of brain tissue were observed by HE staining and real-time polymerase chain reactionation (RT-PCR) was used to detect the expression of Sirt1, PGC-1 伪 and FOXO3 伪 m RNA in hippocampal tissue. Western blotting assay was used to detect the expression of silencing signal regulating factor-2 related enzymes I(Silent information adjustment factor 2 related enzyme sirt1 and cysteiny1 aspartate-specific protein caspase3 in hippocampus. Superoxide dismutase (SOD) and malondialdehyde (Malonaldehydede MDA) kit were used to detect the activity of MDA in hippocampus homogenate. Morris water maze test was carried out on the 30th day to evaluate the learning and memory ability of the rats in each group. Results: compared with the Con group, the nerve tissue in the Sevo group was obviously loose, edema, partial cytoplasmic dissolution, nuclear pyknosis and fragmentation. After RES was pretreated with sevoflurane in Sevo RES group, cell edema and nuclear pyknosis were also observed, but the degree was much less than that in Sevo group. Compared with Con group, the expression of P0.05 Con 伪 -FOXO 3 伪 m RNA in Sevo group decreased the activity of P0.05, the MDA level increased in Sevo RES group and the expression of FOXO3 伪 m RNA in Sevo RES group was significantly higher than that in Sevo group. The results of Western blot showed that the Sirt1 expression in Sevo group and Sevo RES group was significantly higher than that in Sevo RES group. The results of Western blot showed that the expression of Sirt1 in Sevo RES group was significantly higher than that in Sevo RES group, and the expression of FOXO3 伪 m RNA in Sevo RES group was significantly higher than that in Sevo RES group. The results of Western blot showed that the expression of Sirt1 in Sevo group and Sevo RES group was significantly higher than that in Sevo RES group. Compared with Con group, the expression of Sirt1 in Sevo group was significantly higher than that in Sevo group, but the expression of Caspase3 in Sevo RES group was lower than that in Sevo group. Morris water maze test indicated that the escape latency was increased in each group. Conclusion: resveratrol can reduce oxidative stress level and reduce brain damage induced by sevoflurane exposure in neonatal SD rats. Sevoflurane had no significant damage to the long-term learning and memory ability of SD rats.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R614

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