胎盘羊膜对兔胆道损伤后早期修复过程中α-SMA表达的影响

发布时间：2018-04-01 01:04

本文选题：胆管损伤　切入点：炎症　出处：《遵义医学院》2017年硕士论文

【摘要】：目的:研究脱细胞羊膜对兔胆管损伤早期修复过程中α-SMA表达的影响以推断脱细胞羊膜是否在胆道瘢痕的修复方面有促进作用,并与丹参干预效果进行比较。方法:取新鲜胎盘羊膜,经过清洗、分离、浸泡等脱细胞处理后用于实验组干预。将52只兔使用随机数字表法分成4组:假手术组(A组),模型组(B组),阳性对照组(C组)及实验组(D组)。A组兔开腹后游离胆管,关腹。B、C、D组使用钳夹法建立兔胆管损伤模型,B组、C组关腹,脱细胞羊膜覆盖D组胆管损伤处,C组术后第2日开始经静脉注射丹参注射液,每日1次,连续1周。B组、D组术后第2日开始经静脉注射同体积的生理盐水,每日1次,持续1周。在干预完成后2周、4周、8周、12周四个时间点分别取材,使用HE染色法观察胆管损伤处组织的炎症反应轻重及组织形态变化情况;通过免疫组化法、Western Blot法检测4组在四个不同时间点α-SMA的表达量。结果:1.大体和镜下观察:A组:无胆管狭窄,可见组织粘连,局部有炎症细胞浸润,B、C组造模后均出现:胆管损伤处下端狭窄、上端胆管扩张,周围组织粘连,局部组织结构破坏、细胞坏死、炎症细胞浸润、组织增生等情况,D组:胆管损伤处下端狭窄、上端胆管扩张及组织粘连较轻,有局部组织结构破坏、细胞坏死、炎症细胞浸润、组织增生。在同一时间点,D组的炎症反应程度较B组轻。2.在蛋白水平检验胆管损伤组织中α-SMA的表达:在相同时间点下,D组的α-SMA表达水平低于C组(P0.05),C组α-SMA表达水平低于B组(P0.05);A组α-SMA的表达水平无明显变化(P0.05);B、C、D各组α-SMA的表达水平随时间推移而增加(P0.05)。结论:1.在胆管损伤后早期瘢痕修复过程中,α-SMA表达水平随时间推移而增加;2.脱细胞羊膜固定覆盖受损胆管处可以降低损伤处炎症反应程度及α-SMA的表达水平;3.相对于丹参,脱细胞羊膜对瘢痕形成的干预效果更佳
[Abstract]:Aim: to study the effect of acellular amniotic membrane on 伪 -SMA expression during early repair of bile duct injury in rabbits, and to infer whether acellular amniotic membrane can promote the repair of bile duct scar, and to compare the effect of acellular amniotic membrane with Salvia miltiorrhiza.Methods: the fresh placenta amniotic membrane was washed, separated and soaked.52 rabbits were randomly divided into 4 groups: sham operation group (group A), model group (group B), positive control group (group C) and experimental group D group (group A).The model of bile duct injury in rabbits was established by clamp method. The amniotic membrane was used to cover the bile duct injury in group D on the 2nd day after operation, the injection of Salvia miltiorrhiza was injected intravenously, once a day.Group D received intravenously the same volume of normal saline once a day for 1 week.After 2 weeks, 4 weeks, 8 weeks and 12 days, samples were collected, and the severity of inflammatory reaction and the changes of tissue morphology were observed by HE staining.The expression of 伪 -SMA in 4 groups was detected by immunohistochemical method and Western Blot method at four different time points.The result is 1: 1.Gross and microscopic observation in group A: no bile duct stricture, visible tissue adhesion, local inflammatory cell infiltration. All the models appeared in group C: stricture at the lower end of bile duct injury, dilatation of upper bile duct, adhesion of surrounding tissue, destruction of local tissue structure.Cell necrosis, inflammatory cell infiltration and tissue proliferation were found in group D: stricture at the lower end of bile duct injury, slight dilatation and adhesion of upper bile duct, destruction of local tissue structure, necrosis of cells, infiltration of inflammatory cells, and tissue proliferation.The degree of inflammation in group D was lighter than that in group B at the same time.The expression of 伪 -SMA in bile duct injury tissue was detected at the same time: the expression level of 伪 -SMA in group D was lower than that in group C (P 0.05) and group C was lower than that in group B (P 0.05). There was no significant change in the expression of 伪 -SMA in group A (P 0.05).The level of P0. 05% increased with time.Conclusion 1.During the early scar repair after bile duct injury, 伪 -SMA expression increased with time.Acellular amniotic membrane fixation covering the damaged bile duct could reduce the degree of inflammation and the expression level of 伪 -SMA.Compared with Salvia miltiorrhiza, the effect of acellular amniotic membrane on scar formation is better.
【学位授予单位】：遵义医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R657.4

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