川芎嗪对神经病理性疼痛大鼠背根神经节GAP-43mRNA表达的影响

发布时间：2018-04-02 07:20

本文选题：川芎嗪　切入点：神经痛　出处：《兰州大学》2015年硕士论文

【摘要】：目的：评价川芎嗪对慢性坐骨神经压迫性损伤大鼠的脊髓背根神经节GAP-43 mRNA表达的影响,初步探讨川芎嗪对神经病理性疼痛大鼠的作用和相关的机制。方法：选取成年雄性健康Wistar大鼠共54只,周龄6～8周,体重180～220 g。分组方法采用随机数字表法,即将54只大鼠分为3组(n=18)：假手术组(S组)、神经病理性疼痛组(NP组)和川芎嗪组(TMP组)。NP组和TMP组采用慢性坐骨神经压迫损伤法建立神经病理性疼痛模型,S组大鼠仅暴露坐骨神经不结扎。TMP组于CCI术毕即刻腹腔注射川芎嗪100mg·kg-1,1天1次,而S组和NP组给予等容量生理盐水腹腔注射,连续给药至处死当天。S组、NP组和TMP组分别随机取6只大鼠,于术前1 d,术后3、7、14 d(T0～3)时测定机械痛阈(MWT)和热痛阂(TWL),并于T1-3时测定痛阈后处死,取术侧L4-L6背根神经节(DRG),采用实时荧光PCR法检测生长相关蛋白(GAP-43)mRNA的表达水平。结果：与S组比较,NP组和TMP组MWT和TWL在T1-3时降低,背根神经节GAP-43 mRNA表达上调(P0.05)。与NP组比较,TMP组MWT和TWL在T1-3时升高,背根神经节GAP-43 mRNA表达下调(P0.05)。与T1时比较,NP组和TMP组MWT和TWL在T2-3时升高,背根神经节GAP-43mRNA表达下调(P0.05)。结论：川芎嗪减轻大鼠神经病理性疼痛的机制可能与其抑制脊髓背根神经节GAP-43 mRNA表达上调有关。
[Abstract]:Aim: to evaluate the effect of ligustrazine on the expression of GAP-43 mRNA in spinal dorsal root ganglion (DRG) of rats with chronic sciatic nerve compression injury, and to explore the effect of ligustrazine on neuropathic pain in rats.Methods: 54 adult male healthy Wistar rats were selected.The grouping method is random digital table,54 rats were divided into 3 groups: sham operation group (S group), neuropathic pain group (NP group), tetramethylpyrazine group (TMP group) and TMP group (using chronic sciatic nerve compression injury) to establish neuropathic pain model rats in S group.In the unligated sciatic nerve group, ligustrazine 100mg kg-1 was injected intraperitoneally immediately after CCI.Group S and group NP were intraperitoneally injected with normal saline of equal volume, and 6 rats were randomly selected from group S and group TMP, respectively, on the day of death.The mechanical pain threshold (MWTT) and thermal pain barrier (TWL) were measured 1 day before operation and 7 days after operation, and the pain threshold was killed at T1-3. The expression of growth associated protein (GAP-43) was detected by real-time fluorescence PCR method in the dorsal root ganglion (DRG) of the dorsal root ganglion of L4-L6.Results: compared with S group, MWT and TWL in NP group and TMP group were decreased at T1-3, and GAP-43 mRNA expression was up-regulated in dorsal root ganglion (DRG).Compared with NP group, MWT and TWL in TMP group increased at T1-3 and the expression of GAP-43 mRNA in DRG was down-regulated (P 0.05).Compared with T1, MWT and TWL in NP group and TMP group were increased at T2-3, and the expression of GAP-43mRNA in dorsal root ganglion was down-regulated (P 0.05).Conclusion: the mechanism of ligustrazine in relieving neuropathic pain may be related to the up-regulation of GAP-43 mRNA expression in dorsal root ganglion of spinal cord.
【学位授予单位】：兰州大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R614

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