孕早期母体丙泊酚暴露对子代学习记忆和组蛋白去乙酰化酶的影响

发布时间：2018-04-05 21:17

本文选题：丙泊酚　切入点：妊娠　出处：《医学研究生学报》2017年08期

【摘要】：目的孕期母体丙泊酚暴露对子代学习记忆有明显的损害作用,然而其具体机制尚未明确。文中旨在探讨孕早期母体丙泊酚暴露对子代大鼠学习记忆功能以及组蛋白去乙酰化酶2(HDAC2)的影响。方法取孕5~7 d的SD大鼠20只,采用随机数表法随机分为对照组(等渗盐水)和丙泊酚组(丙泊酚),每组10只。子鼠出生后,丙泊酚组每只母鼠所产子鼠分为丙泊酚SAHA组(n=50,注射90 mg/kg HDAC抑制剂SAHA)和丙泊酚溶媒组(n=47,注射等量溶媒DMSO);对照组每只母鼠所产子鼠分为对照SAHA组(n=48,注射90 mg/kg SAHA)和对照溶媒组(n=45,注射等量溶媒DMSO)。各组进行Morris水迷宫实验。处死后取海马组织,采用免疫荧光法检测HDAC2蛋白的表达水平。结果丙泊酚溶媒组水迷宫实验第5、6天子鼠逃避潜伏期[(65.93±30.42)、(50.72±24.72)s]较对照溶媒组[(29.32±16.38)、(21.34±13.79)s]、丙泊酚SAHA组[(42.52±20.43)、(24.28±13.41)s]明显延长,穿越平台次数减少(P0.05)。丙泊酚溶媒组HDAC2蛋白表达水平(1.37±0.03)较对照溶媒组(1.00±0.02)、丙泊酚SAHA组(1.08±0.02)明显升高(P0.05)。结论孕早期丙泊酚暴露可引起子代大鼠学习记忆功能损害,其机制与上调子代大鼠海马区HDAC2蛋白表达有关。
[Abstract]:Objective Maternal propofol exposure has a significant effect on learning and memory in offspring, but its mechanism has not been clarified.The purpose of this study was to investigate the effects of maternal propofol exposure on learning and memory function and histone deacetylase 2 (HDAC2) in offspring.Methods Twenty SD rats were randomly divided into control group (isotonic saline) and propofol group (10 rats in each group).After birth,The solvent group was injected with the same amount of solvent DMSON.Morris water maze test was carried out in each group.The expression of HDAC2 protein was detected by immunofluorescence.The expression level of HDAC2 protein in propofol group (1.37 卤0.03) was significantly higher than that in control group (1.00 卤0.02) and propofol SAHA group (1.08 卤0.02).Conclusion Propofol exposure in early pregnancy may induce learning and memory impairment in offspring rats, and its mechanism may be related to up-regulation of HDAC2 protein expression in hippocampus of offspring rats.
【作者单位】：南昌大学第一附属医院麻醉科;中山大学附属第一医院东院麻醉科;
【基金】：国家自然科学基金(81460175)
【分类号】：R614

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