塞来昔布对颅脑创伤后Bcl-2表达及学习记忆功能的影响

发布时间：2018-04-09 10:19

本文选题：颅脑损伤　切入点：环氧化酶-　出处：《安徽医科大学学报》2017年10期

【摘要】：目的探讨选择性环氧合酶(COX-2)抑制剂塞来昔布对大鼠颅脑创伤后B淋巴细胞瘤-2基因(Bcl-2)表达及学习记忆功能的影响。方法实验分为对照组、假手术组、脑创伤组、治疗组,首先采用Marmarou方法建立大鼠闭合性颅脑创伤模型,qPCR法检测mRNA表达量,免疫组织化学法检测蛋白表达水平,TUNEL染色法检测细胞凋亡,Morris水迷宫实验测试学习记忆功能。结果脑创伤组COX-2的表达明显高于其他3组(P0.05),治疗组较脑创伤组能有效降低COX-2的表达(P0.05);而脑创伤组Bcl-2的表达明显低于其他3组(P0.05),治疗组与脑创伤组比较能有效增高Bcl-2的表达(P0.05);脑创伤组TUNEL染色阳性细胞数均高于其它各组(P0.05),治疗组相较于脑创伤组能显著降低阳性细胞数(P0.05);脑创伤组搜索平台所需的时间大于其他3组(P0.05),治疗组较脑创伤组能有效降低搜索平台所需时间(P0.05)。结论塞来昔布通过对COX-2的特异性抑制,减轻颅脑创伤后的炎症反应,增加Bcl-2的表达,从而抑制细胞的凋亡,发挥脑保护作用,并改善其脑创伤后的学习记忆功能障碍。
[Abstract]:Objective to investigate the effects of celecoxib, a selective cyclooxygenase inhibitor, on the expression of Bcl-2 gene and the learning and memory function of B lymphocytoma in rats after traumatic brain injury (TBI).Methods the experiment was divided into three groups: control group, sham operation group, brain trauma group and treatment group. The expression of mRNA was detected by qPCR in closed traumatic brain injury model of rats established by Marmarou method.Immunohistochemical method was used to detect the expression of protein and Tunel staining was used to detect apoptosis and Morris water maze test to test the function of learning and memory.Results the expression of COX-2 in the brain trauma group was significantly higher than that in the other three groups (P 0.05). The expression of Bcl-2 in the treatment group was significantly lower than that in the brain trauma group, but the expression of Bcl-2 in the brain trauma group was significantly lower than that in the other three groups. The expression of Bcl-2 in the treatment group was significantly higher than that in the brain trauma group.The expression of Bcl-2 and the number of TUNEL staining positive cells in the brain trauma group were higher than those in the other groups. Compared with the brain trauma group, the number of positive cells in the treatment group was significantly lower than that in the brain trauma group, and the time required for searching the platform in the trauma group was longer than that in the other three groups, and the treatment group was higher than that in the treatment group.The brain trauma group could effectively reduce the time required for searching platform (P 0.05).Conclusion Celecoxib can attenuate the inflammatory reaction after traumatic brain injury and increase the expression of Bcl-2 through the specific inhibition of COX-2, thus inhibiting the apoptosis of cells, exerting the brain protection effect, and improving the learning and memory dysfunction after brain trauma.
【作者单位】：河北医科大学附属邢台人民医院神经外科;邢台医学高等专科学校;华北理工大学基础医学院;
【基金】：河北省自然科学基金(编号:C2004000689) 河北省博士基金项目(编号:04276135) 河北省科学技术与社会发展计划项目(编号:05547008D-4)
【分类号】：R651.15

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