基于损伤面积控制大鼠胰腺创伤模型的建立及意义

发布时间：2018-04-10 07:25

本文选题：胰腺创伤模型　切入点：损伤面积　出处：《山东医药》2017年25期

【摘要】：目的探讨建立一种新型大鼠单纯胰腺创伤模型,并基于损伤面积评估其伤情变化。方法将大鼠随机分为2组,采用自主研发多功能撞击仪的3 cm~2(3 cm~2致伤面积组)和6 cm~2(6 cm~2致伤面积组)撞击头,分别挤压各组处于开腹状态的胰腺,使之在400 k Pa的压强下形成不同面积的损伤,并设置对照组;建模24 h后观察各组大鼠存活率、一般情况、大体病理和组织病理学改变,并检测血钾、血钙的浓度和血清、腹水中淀粉酶及脂肪酶的活性。结果与对照组比较,3 cm~2和6 cm~2致伤面积组均能形成明显的损伤(P0.05),其中,与3 cm~2致伤面积组比较,6 cm~2致伤面积组损伤更显著(P0.05)。与3 cm~2致伤面积组相比,6 cm~2致伤面积组大鼠存活率、血钾及血钙水平更低(P均0.05),一般情况更差,损伤性病理改变的范围及程度更大,血清淀粉酶、脂肪酶更高(P均0.05)。结论成功建立了一种基于损伤面积控制的大鼠胰腺创伤模型。该模型简单、有效、可控性及可重复性好,适合用于伤情演化机制和创伤施救等方面的研究。
[Abstract]:Objective to establish a new simple pancreatic trauma model in rats and to evaluate the injury status based on the injury area.Methods the rats were randomly divided into two groups. The rats were randomly divided into two groups. The rats were randomly divided into two groups. The rats were randomly divided into two groups: the 3 cm~2(3 cm~2 injury area group (3 cm~2(3 cm~2 group) and the 6 cm~2(6 cm~2 injury area group (6 cm~2(6 cm~2 group).After 24 hours of modeling, the survival rate, general condition, gross pathological and histopathological changes of rats were observed, and the concentrations of blood potassium, serum calcium and serum were measured.Activities of amylase and lipase in ascites.Results compared with the control group, the injury area of 3 cm~2 and 6 cm~2 could form significant injury P0.05A, among which, compared with the 3 cm~2 group, the injury of 6 cm~2 group was more significant than that of the 6 cm~2 group.Compared with 3 cm~2 injury area group, the survival rate, blood potassium and blood calcium levels of rats in the 6 cm~2 injury area group were lower (P < 0.05), the general condition was worse, the range and extent of pathological changes were larger, the serum amylase and lipase levels were higher than those in the control group (P < 0.05).Conclusion A rat pancreatic trauma model based on area control was successfully established.The model is simple, effective, controllable and reproducible. It is suitable for the study of injury evolution mechanism and wound rescue.
【作者单位】：西南医科大学;成都军区总医院全军普外中心;
【基金】：国家自然科学基金资助项目(81001695) 四川省科技厅应用基础研究项目(2013JY0046)
【分类号】：R-332;R657.5

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