人脐带Wharton’s Jelly间充质干细胞治疗老年肌肉衰减综合征的实验研究

发布时间：2018-04-10 12:17

本文选题：人脐带Wharton’s + Jelly间充质干细胞　；参考：《青岛大学》2017年硕士论文

【摘要】：目的:利用人脐带Wharton’s Jelly间充质干细胞(h UCMSCs)移植治疗老年肌肉衰减综合征模型小鼠,探讨干细胞移植治疗的方法及可行性。方法:利用后肢悬提技术建立老年肌肉衰减综合征模型。取24月龄C57BL/6雄性小鼠24只,随机分为静脉注射干细胞组、肌肉注射干细胞组、静脉注射汉克氏液组、肌肉注射汉克氏液组,每组6只。用胰酶消化法与差速贴壁法培养h UCMSCs,传代3次后分别利用腓肠肌局部和尾静脉注射的方法进行h UCMSCs移植治疗。通过比较最大等距跖屈肌力、腓肠肌的肌肉湿重、肌纤维的横截面积、I型肌肉纤维的占比等指标对h UCMSCs移植的疗效进行评价。结果:1.胰酶消化与差速贴壁法培养h UCMSCs,可获得稳定传代及足量的细胞,能够保证实验的需求。2.后肢悬提2周导致老年小鼠的体重下降,小鼠重新行走并接受h UCMSCs移植8周后,体重仍继续下降。3.后肢悬提可以显著降低老年小鼠最大等距跖屈肌力,h UCMSCs移植可以显著改善老年小鼠的最大等距跖屈肌力,局部肌肉内h UCMSCs移植效果要好于尾静脉注射移植。4.h UCMSCs移植显著增加腓肠肌的湿重、肌纤维的横截面积及腓肠肌肌肉组织中I型肌肉纤维的占比,局部肌肉注射移植同样优于尾静脉注射移植。结论:1.hUCMSCs易于体外培养扩增,可以通过体外注射进行细胞移植2.hUCMSCs移植可显著增加腓肠肌的湿重,并显著改善老年小鼠的下肢肌力,提示h UCMSCs移植在老年肌肉衰减综合征模型动物体内能够起到一定的治疗作用。
[Abstract]:Objective: to study the feasibility of transplantation of Wharton's Jelly mesenchymal stem cells from human umbilical cord to treat senile muscle attenuation syndrome model mice.Methods: the model of senile muscle attenuation syndrome was established by hind limb suspension technique.Twenty-four C57BL/6 male mice aged 24 months were randomly divided into three groups: intravenous stem cell group, intramuscular stem cell injection group, Hank's fluid group and Hank's fluid group.HUCMSCs were cultured with trypsin digestion and differential adherent method. After three passages, the local and caudal vein injection of gastrocnemius was used for the transplantation of h UCMSCs respectively.The effect of h UCMSCs transplantation was evaluated by comparing the maximum isometric flexor muscle strength, muscle wet weight of gastrocnemius muscle, and the ratio of muscle fiber cross-sectional area to type I muscle fiber.The result is 1: 1.By trypsin digestion and differential adherence method, stable passage and sufficient number of cells could be obtained.The hind limb suspension for 2 weeks resulted in the weight loss of the aged mice, and the weight loss continued to decrease 8 weeks after the mice walked again and received h UCMSCs transplantation for 8 weeks.The hind limb suspension could significantly reduce the maximal isometric flexor muscle strength of the aged mice and the maximum isometric flexor muscle strength of the aged mice could be significantly improved by the transplantation of h UCMSCs.The effect of local intramuscular h UCMSCs transplantation was better than that of tail vein injection. 4. H UCMSCs transplantation significantly increased the wet weight of gastrocnemius muscle, the cross sectional area of muscle fiber and the proportion of type I muscle fiber in gastrocnemius muscle tissue.Local intramuscular injection was also superior to caudal vein transplantation.Conclusion\\\%\%\%\%\%\%\%\%\%\%\%\%\%\%\%\%\%\%\%\%\%\%\%\%\% UCMSCs can be easily cultured and amplified in vitro. 2.hUCMSCs transplantation can significantlyThese results suggest that h UCMSCs transplantation can play a therapeutic role in senile muscle attenuation syndrome model animals.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R685

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