褪黑素对急性脊髓损伤大鼠脊髓组织中MPO及CINC-1表达的影响

发布时间：2018-04-11 00:09

本文选题：褪黑素 + 脊髓损伤　；参考：《郑州大学学报(医学版)》2016年06期

【摘要】：目的:探讨褪黑素(MT)对大鼠急性脊髓损伤后髓过氧化物酶(MPO)及中性粒细胞趋化因子(CINC)-1表达的影响。方法:108只SD大鼠分为假手术组、脊髓损伤组、褪黑素治疗组。手术组大鼠仅切除T11椎板,脊髓损伤组、褪黑素治疗组建立T11脊髓损伤模型。脊髓损伤组、褪黑素治疗组在脊髓损伤后10 min内分别腹腔注射含体积分数5%乙醇的生理盐水和100 mg/kg的褪黑素制剂。于脊髓损伤后1 h、6 h、12 h、1 d、3 d、5 d分别取脊髓组织,化学法测定MPO活力变化,HE染色法观察脊髓组织结构,RT-PCR法观察CINC-1 mRNA表达情况。结果:脊髓内CINC-1水平和MPO活性有相似的变化规律,且MPO在CINC-1表达达峰之后12 h达高峰;除1 h外各时间点各组相比较,B组最高、C组次之、A组最低,其中B组与A组、C组差异具有统计学意义(P0.05)。结论:褪黑素可通过抑制CINC-1的表达减少中性粒细胞的浸润,从而减轻炎症反应,起到一定的神经保护作用。
[Abstract]:Aim: to investigate the effects of melatonin (MT) on the expression of myeloperoxidase (MPO) and neutrophil chemokine (CINC- 1) after acute spinal cord injury (sci) in rats.Methods 108 Sprague-Dawley rats were divided into sham operation group, spinal cord injury group and melatonin treatment group.T11 spinal cord injury model was established in the operation group, spinal cord injury group and melatonin treatment group.Spinal cord injury group and melatonin treatment group were intraperitoneally injected with 5% ethanol saline and 100 mg/kg melatonin preparation 10 min after spinal cord injury.The spinal cord tissue was taken from the spinal cord at 1 h, 12 h, 12 h, 3 d and 5 d after spinal cord injury. The activity of MPO was determined by chemical method. The structure of spinal cord was observed by HE staining. The expression of CINC-1 mRNA was detected by RT-PCR.Results: the level of CINC-1 and the activity of MPO in spinal cord showed similar changes, and MPO reached its peak 12 hours after the peak of CINC-1 expression.The difference between group B and group A was statistically significant (P 0.05).Conclusion: melatonin can reduce the infiltration of neutrophils by inhibiting the expression of CINC-1, thus lighten the inflammatory reaction and play a neuroprotective role.
【作者单位】：郑州大学第一附属医院;郑州大学第一附属医院骨科;
【基金】：河南省国际科技合作项目134300510005
【分类号】：R651.2

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