盐酸氨基葡萄糖对膝骨关节炎膝关节软骨下骨Ⅰ型胶原及骨钙素表达的影响

发布时间：2018-04-11 08:28

本文选题：Ⅰ型胶原 + 骨钙素　；参考：《郑州大学学报(医学版)》2017年01期

【摘要】：目的:探讨盐酸氨基葡萄糖对膝骨关节炎膝关节软骨下骨Ⅰ型胶原及骨钙素表达的影响。方法:选取46例因膝关节骨性关节炎行人工膝关节表面置换术的绝经后女性患者,其中23例术前半年内行盐酸氨基葡萄糖治疗12周,为观察组,未服用者23例为对照组。术中留取2组膝关节胫骨平台截骨后软骨下骨组织0.5 cm3,分别行骨组织显微CT检测、Ⅰ型胶原胶原苦味酸-天狼星红染色、骨钙素免疫组化染色、Ⅰ型胶原及骨钙素Western blot检测。结果:经显微CT检测发现,观察组软骨下骨小梁相对骨体积、骨小梁间隙、骨小梁厚度、骨小梁数目均较对照组有所改善,且观察组Ⅰ型胶原和骨钙素含量均较对照组增加(P0.05)。Western blot检测亦证实观察组软骨下骨Ⅰ型胶原及骨钙素表达较对照组增多(P0.05)。结论:盐酸氨基葡萄糖可能通过调节Ⅰ型胶原及骨钙素的表达,从而改善女性膝关节骨性关节炎绝经患者膝关节软骨下骨的微结构,进而改善膝关节软骨下骨功能。
[Abstract]:Objective: To investigate the effect of glucosamine hydrochloride on the expression of type I collagen and osteocalcin in bone of knee osteoarthritis of knee articular cartilage. Methods: 46 cases with postmenopausal knee osteoarthritis treated with artificial knee arthroplasty after female patients, including 23 cases of the first half of the year within 12 weeks of treatment, acid salt of glucosamine. For the observation group, not taking 23 cases as control group. 2 groups of intraoperative specimens of tibial osteotomy after subchondral bone tissue were performed in 0.5 cm3, bone collagen type I collagen CT detection, picrosirius red staining, osteocalcin immunohistochemical staining, collagen and osteocalcin Western blot detection. Results: the microscopic CT detection showed that the observation group of subchondral bone trabecular bone volume, trabecular bone Liang Jianxi, trabecular thickness, trabecular number significantly improved, and the observation group of type I collagen and osteocalcin content were compared with the control Group.Western (P0.05) blot detection also confirmed the observation of collagen type I and osteocalcin bone under the cartilage expression group was more than that in control group (P0.05). Conclusion: Glucosamine Hydrochloride might regulate the expression of type I collagen and osteocalcin, thereby improving the micro structure of women with knee osteoarthritis in postmenopausal patients with knee joint subchondral bone. In order to improve the function of knee joint subchondral bone.

【作者单位】：西安交通大学第二附属医院骨一科;
【基金】：国家自然科学基金资助项目81301562 西安交通大学第二附属医院人才培养专项科研基金RC(XM)201501
【分类号】：R684.3

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