柴胡皂苷a对大鼠急性脊髓损伤的神经保护作用与机制研究

发布时间：2018-04-11 15:25

本文选题：柴胡皂苷a + 脊髓损伤　；参考：《中国修复重建外科杂志》2017年07期

【摘要】：目的探讨柴胡皂苷a(saikosaponin a,SSa)对大鼠急性脊髓损伤早期机体免疫炎性水平的影响,并研究其可能存在的影响机制。方法取健康成年雌性SD大鼠72只,体质量220~250 g,随机分为假手术组(A组)、脊髓损伤组(B组)、SSa处理组(C组),每组24只。A组仅咬除棘突和椎板暴露脊髓;B、C组利用改良Allen重物打击法制作急性脊髓损伤模型,造模后即刻C组给予大鼠腹腔注射10 mg/kg SSa,B组注入等量生理盐水。术后24 h每组处死18只大鼠并取出脊髓组织,采用ELISA法检测TNF-α和IL-6浓度,Western blot检测NF-κB P65、NF-κB P-P65和水通道蛋白4(aquaporin 4,AQP4)蛋白表达水平,HE染色观察脊髓组织形态;每组其余6只大鼠分别于术后1、3、7、14、21、28 d采用BBB评分和斜板实验评价大鼠双下肢运动恢复情况。结果术后各时间点A组BBB评分和斜板实验最大角度均显著高于B、C组(P0.05),术后14、21、28 d C组上述指标显著高于B组(P0.05)。ELISA检测示,A组TNF-α、IL-6浓度显著低于B、C组,C组显著低于B组,差异均有统计学意义(P0.05)。Western blot检测示,A组NF-κB P65、NF-κB P-P65和AQP4蛋白表达水平显著低于B、C组,C组显著低于B组,差异均有统计学意义(P0.05)。HE染色示,A组脊髓中神经细胞正常,无明显病变;B组脊髓中损伤部位可见神经元细胞,损伤处有出血、中性粒细胞浸润、神经细胞水肿;C组脊髓中可见神经元细胞,小胶质细胞轻度增生,病变较B组好转。结论 SSa通过抑制NF-κB信号通路和AQP4蛋白的表达,减轻急性脊髓损伤后机体炎性反应和组织水肿,从而具有一定保护神经的功能。
[Abstract]:Objective to investigate the effect of saikosaponin a(saikosaponin a sac on the level of immune inflammation in the early stage of acute spinal cord injury (sci) in rats and its possible mechanism.Methods 72 healthy adult female SD rats were selected.Immediately after modeling, rats in group C were intraperitoneally injected with normal saline for 10 mg/kg.At 24 hours after operation, 18 rats in each group were killed and spinal cord tissues were removed. The expression levels of NF- 魏 B P65, NF- 魏 B P-P65 and aquaporin 4(aquaporin 4 AQP4) protein were detected by ELISA and IL-6. The morphology of spinal cord was observed by HE staining.Neuron cells were found in the injured part of spinal cord in group B, hemorrhage and neutrophil infiltration were found in the injured area. Neurons were found in spinal cord in group C with neuronal edema and mild proliferation of microglia. The lesion was better than that in group B.Conclusion SSa can attenuate inflammatory reaction and tissue edema after acute spinal cord injury by inhibiting NF- 魏 B signaling pathway and the expression of AQP4 protein.
【作者单位】：天津医科大学研究生院;天津医科大学总医院骨科;武警后勤学院附属医院脑科中心;武警后勤学院细胞生物学教研室;武警后勤学院附属医院救援研究所;
【基金】：国家科技重点研发计划资助项目(2016YFC1101500) 国家自然科学基金面上项目(11672332);国家自然科学基金青年项目(81101362)~~
【分类号】：R651.2

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