大鼠ALPPS术后残肝再生与Wnt2蛋白表达关系的研究

发布时间：2018-04-13 20:02

本文选题：肝再生 + ALPPS　；参考：《安徽医科大学学报》2017年09期

【摘要】：目的探讨大鼠联合肝脏分割和门静脉结扎分阶段肝切除术(ALPPS)后残余肝再生Wnt2蛋白的表达变化,进一步了解肝再生过程中的分子机制。方法选取200~240g健康SD雄性大鼠40只,随机分为ALPPS组和假手术(Sham)组;Sham组仅游离出门静脉各分支,不结扎即关腹;ALPPS组行大鼠肝左外叶、左中叶、右叶门静脉分支结扎,切除尾状叶,保留肝右中叶分支,并行肝中叶实质离断。分别于术后1、2、4、7 d处死5只大鼠,称取大鼠肝右中叶重量,计算肝再生率;光学显微镜下观察肝右中叶细胞的形态学变化,免疫组化法检测肝右中叶Ki-67和Wnt2蛋白表达变化,并进行统计分析。结果 (1)ALPPS术后保留侧肝脏开始再生,第2天肝再生率升高最快,随后肝再生率升高速度逐渐降低,第7天与Sham组相比肝再生率升高速度两者相近;(2)ALPPS术后Ki-67与Sham组比较,第1天开始升高,第2天达到最高,此后逐渐降低,第7天有少量阳性细胞,与Sham组相比差异无统计学意义;(3)Wnt2阳性表达主要在中央静脉周围,可能与肝干细胞的产生相关;与Sham组相比,肝右中叶Wnt2蛋白在ALPPS术后第1天即开始升高,第2天达到最高,然后逐渐降低,第7天时仅有少量表达,与Sham组相比差异无统计学意义;(4)大鼠肝脏ALPPS术后Wnt2的表达与术后Ki-67的表达呈正相关性。结论 Wnt2在ALPPS术后肝脏再生及肝干细胞表达过程中起重要的调控作用。
[Abstract]:Objective to investigate the expression of Wnt2 protein in residual liver regeneration after combined liver segmentation and portal vein ligation in rats, and to further understand the molecular mechanism of liver regeneration.Methods Forty healthy male SD rats were randomly divided into ALPPS group and sham group. All branches of portal vein were isolated from the portal vein in the sham group. The left lateral lobe, left middle lobe and right lobe of the rat liver were ligated and caudate lobes were resected without ligation of the left lateral lobe of liver, left middle lobe and right lobe.The right middle lobe branch was preserved and the middle lobe parenchyma was cut off.On the 7th day after operation, 5 rats were killed, and the weight of the right middle lobe of liver was taken out to calculate the rate of liver regeneration, the morphological changes of the cells in the right middle lobe of the liver were observed under optical microscope, and the expression of Ki-67 and Wnt2 protein in the right middle lobe of the liver were detected by immunohistochemical method.Statistical analysis was carried out.Results liver regeneration began to occur in the reserved side after ALPPS operation, and the rate of liver regeneration increased fastest on the second day, and then decreased gradually. On the 7th day, the rate of liver regeneration in the Ki-67 group was similar to that in the Sham group compared with that in the Sham group.On the first day, the expression of Wnt2 increased, reached its peak on the second day, then decreased gradually. On the 7th day, there were a small number of positive cells. There was no significant difference between the Sham group and the Sham group. The positive expression of WNT2 was mainly around the central vein, which might be related to the production of hepatic stem cells.Compared with Sham group, Wnt2 protein in right middle lobe of liver began to increase on the first day after ALPPS, reached the highest level on the second day, then decreased gradually, and only a small amount of protein was expressed on the 7th day.The expression of Wnt2 after ALPPS was positively correlated with the expression of Ki-67 in the liver of rats compared with Sham group.Conclusion Wnt2 plays an important role in regulating liver regeneration and hepatic stem cell expression after ALPPS.
【作者单位】：安徽医科大学无锡临床学院肝胆外科;中国人民解放军第一0一医院肝胆外科;
【基金】：南京军区医学科技创新课题重点资助项目(编号:14D05)
【分类号】：R657.3

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